| 张海容，陈金娥，高瑞苑.响应面法优化超声提取芝麻粕中多酚工艺及其抗氧化活性研究[J].中国油脂,2019,44(1):.[ZHANG Hairong, CHEN Jin e, GAO Ruiyuan.Optimization of ultrasound-assisted extraction of polyphenols from sesame meal by response surface methodology and its antioxidant activity[J].China Oils and Fats,2019,44(1):.] |
| 响应面法优化超声提取芝麻粕中多酚工艺及其抗氧化活性研究 |
| Optimization of ultrasound-assisted extraction of polyphenols from sesame meal by response surface methodology and its antioxidant activity |
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| DOI： |
| 中文关键词: 芝麻粕 多酚 超声提取 响应面 抗氧化 |
| 英文关键词:sesame meal polyphenols ultrasound-assisted extraction response surface methodology antioxidation |
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| 中文摘要: |
| 采用超声辅助提取芝麻粕中多酚。通过单因素试验考察提取溶剂、超声功率、超声温度、液料比、超声时间对多酚产率的影响，在此基础上，基于Box-Behnken进行了四因素三水平响应面优化设计，并测定了芝麻粕多酚提取物体外抗氧化活性。结果表明：超声辅助提取芝麻粕中多酚的最佳提取条件为以60%乙醇溶液为提取溶剂、超声功率320 W、超声温度54?℃、液料比57∶?1、超声时间30 min，此条件下多酚产率为2.86%，与预测值（2.95%）无显著性差异。与食品抗氧化剂TBHQ相比，芝麻粕多酚提取物具有较强的体外抗氧化活性，芝麻粕多酚提取液清除DPPH·的能力（IC50为34.91 μg/mL）大于TBHQ的（IC50为92.40 μg/mL）。 |
| 英文摘要: |
| Polyphenols was extracted from sesame meal by ultrasound-assisted technology. The effects of solvent, ultrasonic power, ultrasonic temperature, ratio of liquid to material and ultrasonic time on the yield of polyphenols were studied by single factor experiment.Then based on Box-Behnken design, four-factor and three-level response surface methodology was carried out to optimize the extraction process, and the antioxidant activity of polyphenols in vitro was also determined. The results showed that the optimal ultrasound-assisted extraction conditions of polyphenols from sesame meal were obtained as follows: 60% ethanol aqueous solution as solvent, ultrasonic power 320 W, ultrasonic temperature 54?℃, ratio of liquid to material 57∶?1 and ultrasonic time 30 min. Under these conditions, the yield of polyphenols was 2.86%, which was not significantly different from the predictive value(2.95%). Compared with TBHQ, the polyphenol extracts from sesame meal had stronger antioxidant activity in vitro. The IC50 of polyphenol extracts from sesame meal on scavenging DPPH free radical was 34.91 μg/mL,lower than that of TBHQ(92.40 μg/mL). |
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