纪漫,孙培冬.龙眼核活性脂质的分析及其细胞增殖活性研究[J].中国油脂,2019,44(8):.[JI Man, SUN Peidong.Functional lipids from longan seeds and its cell proliferation activity[J].China Oils and Fats,2019,44(8):.]
龙眼核活性脂质的分析及其细胞增殖活性研究
Functional lipids from longan seeds and its cell proliferation activity
  
DOI:
中文关键词:  龙眼核;植物甾醇;神经酰胺;人皮肤成纤维细胞;人永生化角质形成细胞
英文关键词:longan seed; phytosterol; ceramide; HSF; HaCaT
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纪漫,孙培冬  
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中文摘要:
      研究了龙眼核活性脂质的组成及其细胞增殖活性。以龙眼核为原料,经超声辅助提取、氯仿萃取、硅胶柱层析分离后得到富含植物甾醇和神经酰胺的活性脂质组分CEP-2;定性定量分析脂质中的活性成分,并在细胞水平上考察龙眼核活性脂质对人皮肤成纤维细胞(HSF)、人永生化角质形成细胞(HaCaT)增殖活性的影响。结果表明:CEP-2中主要含有三萜类、神经酰胺类、鞣质及酚类物质,总甾醇含量为518.26 mg/g,神经酰胺含量为2.65 mg/g;CEP-2在质量浓度为40 μg/mL时,HSF细胞的存活率为105.28%;在质量浓度为100 μg/mL时,对HaCaT细胞表现出明显的细胞增殖活性(p<0.001),细胞存活率为114.21%。
英文摘要:
      The composition of functional lipids from longan seeds and its cell proliferation activity were studied. Using longan seeds as raw material, the functional lipids CEP-2 rich in phytosterols and ceramides were obtained by ultrasound-assisted extraction, liquid-liquid partitioning (chloroform), and silica column chromatography. Furthermore, the functional components in lipids were qualitatively and quantitatively analyzed. Finally, the effects of functional lipids on proliferation activity of human skin fibroblasts (HSF) and human immortalized keratinocytes (HaCaT) cell lines were investigated. The results showed that CEP-2 mainly contained triterpenoids, ceramides, tannins and phenols, and the contents of total sterol and ceramide were 518.26 mg/g and 2.65 mg/g respectively. When the mass concentration of CEP-2 was 40 μg/mL, the proliferation rate of HSF cells was 105.28%. However, CEP-2 showed significant cell proliferative activity on HaCaT cells (p<0.001) at the mass concentration of 100 μg/mL, and the proliferation rate was 114.21%.
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