| 孙玉莹.非水相脂肪酶催化合成β-谷甾醇正辛酸酯的研究[J].中国油脂,2020,45(11):94~98.[SUN Yuying.Lipase-catalyzed synthesis of β-sitosterol caprylate in non-aqueous media[J].China Oils and Fats,2020,45(11):94~98.] |
| 非水相脂肪酶催化合成β-谷甾醇正辛酸酯的研究 |
| Lipase-catalyzed synthesis of β-sitosterol caprylate in non-aqueous media |
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| DOI：10.12166/j.zgyz.1003-7969/2020.11.019 |
| 中文关键词: β-谷甾醇 正辛酸 Candida sp.99-125脂肪酶 酯化 |
| 英文关键词:β-sitosterol n-caprylic acid Candida sp.99-125 lipase esterification |
| 基金项目:国家自然科学基金（31401664）；江苏省高等学校自然科学研究面上项目（18KJB550001）；江苏大学高级人才科研启动基金（13JDG070）；江苏高校优势学科建设工程资助项目 |
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| 中文摘要: |
| 首次以Candida sp.99-125脂肪酶为催化剂，以正辛酸为亲油修饰剂，以β-谷甾醇作为植物甾醇的代表，催化合成β-谷甾醇正辛酸酯。通过薄层色谱、核磁共振波谱、差示扫描量热分析、高效液相色谱对产物进行分析与表征。另外，通过单因素试验考察了溶剂种类、反应温度、脂肪酶用量、底物摩尔比、底物浓度、反应时间对转化率的影响。结果表明：合成产物为β-谷甾醇正辛酸酯，β-谷甾醇经与正辛酸酯化后熔点显著降低，有助于拓宽植物甾醇的应用范围，在20 mg/mL Candida sp.99-125脂肪酶为催化剂、5 mL异辛烷、50 mmol/L β-谷甾醇、β-谷甾醇与正辛酸摩尔比1∶ 1、120 mg/mL 3分子筛、45 ℃下反应12 h，转化率可达90%以上。 |
| 英文摘要: |
| With Candida sp.99-125 lipase as a catalyst and n-caprylic acid as an acyl donor,β-sitosterol caprylate was synthesized.The synthesized product was characterized by thin layer chromatography, nuclear magnetic resonance analysis, differential scanning calorimetry, high performance liquid chromatography. In addition, the influences of solvent type, dosage of lipase, reaction temperature, substrate molar ratio, substrate concentration and reaction time on conversion rate were studied by single factor experiment.The results showed that synthesized product was identified as β-sitosterol caprylate, and the melting point of β-sitosterol decreased significantly after esterification with n-caprylic acid, which was helpful to broaden the application range of phytosterol. The conversion rate could reach above 90% under the conditions of 20 mg/mL Candida sp.99-125 lipase, 5 mL isooctane, 50 mmol/L β-sitosterol, molar ratio of β-sitosterol to n-caprylic acid 1∶ 1, 120 mg/mL 3 molecular sieves, reaction at 45 ℃ for 12 h. |
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