张忠.不同提取工艺翅果油抗氧化能力与活性成分的分析[J].中国油脂,2020,45(9):23~29.[ZHANG Zhong.Antioxidant ability and active components of Elaeagnus mollis Diels seed oil[J].China Oils and Fats,2020,45(9):23~29.]
不同提取工艺翅果油抗氧化能力与活性成分的分析
Antioxidant ability and active components of Elaeagnus mollis Diels seed oil
  
DOI:
中文关键词:  翅果油;极性部分;非极性部分;DPPH自由基清除能力;ABTS自由基清除能力;羟基自由基清除能力;PF还原法;活性成分
英文关键词:Elaeagnus mollis Diels seeds oil; polar fraction; non-polar fraction; DPPH· scavenging capacity; ABTS free radical scavenging capacity; hydroxyl radical scavenging capacity; PF reducing power; active component
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作者单位
张忠 1.山西医科大学 人体解剖教研室,太原 030001
2.江南大学 食品学院,江苏 无锡 214122
 
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中文摘要:
      采用DPPH自由基、ABTS自由基、羟基自由基清除能力法和PF还原法测定了不同提取工艺(超临界CO2流体萃取法,有机溶剂提取法,直压冷榨法,直压热榨法和红外热榨法)制得的翅果油、翅果油中极性和非极性部分的抗氧化能力,同时对翅果油中的微量活性成分进行了分析,并对翅果油中微量活性成分含量与其抗氧化能力进行了相关性分析。结果表明,除羟基自由基清除能力外,不同翅果油对DPPH自由基、ABTS自由基清除能力和Fe3+还原能力不同,5种提取工艺所得翅果油的极性部分抗氧化活性最强,全油次之,非极性部分抗氧化活性最弱。在翅果油的极性部分中,红外热榨法的DPPH自由基清除能力最强,为1 080.35 μmolTE/100 g,直压冷榨的羟基自由基清除能力最强,为38 272.82 μmolTE/100 g,有机溶剂提取的ABTS自由基清除能力最强,为 2 000.76 μmolTE/100 g,红外热榨的Fe3+还原能力最强,为2 771.89 μmolTE/100 g。翅果油中微量生物活性成分与抗氧化活性相关性分析表明,翅果油中的生物活性成分含量与4种抗氧化活性指标均呈极显著正相关(p<0.01),γ-生育酚、δ-生育酚和羽扇豆醇是翅果油中重要的抗氧化基础物质。
英文摘要:
      The antioxidant capacity of Elaeagnus mollis Diels seed oil extracted by five different processes (supercritical CO2 fluid extraction method, solvent extraction method, cold pressing method, hot pressing method and infrared hot pressing method) was evaluated by DPPH, ABTS and hydroxyl radical scavenging capacity, and PF reducing power, as well as the antioxidant capacity of the polar and non-polar fractions of the oil. In addition, the trace active components in Elaeagnus mollis Diels seed oil were analyzed, and the correlation analysis of the trace active components content and its antioxidant capacity was carried out. The results showed that except for the hydroxyl radical scavenging capacity, the antioxidant capacities on DPPH and ABTS and PF reducing power varied from the different oils, and the polar fractions of oils obtained by the five processes had the strongest antioxidant capacity, followed by the whole oil, and the non-polar fraction was the weakest. In the polar fraction of Elaeagnus mollis Diels seed oil, infrared hot pressing method had the highest DPPH scavenging capacity (1 080.35 μmolTE/100 g), cold pressing method had the highest hydroxyl radical scavenging capacity (38 272.82 μmolTE/100 g), while solvent extraction had the highest ABTS free radical scavenging capacity (2 000.76 μmolTE/100 g), and infrared hot pressing method had the highest Fe3+ reducing power (2 771.89 μmolTE/100 g). The correlation analysis showed that the bioactive components in Elaeagnus mollis Diels seed oil were significantly and positively correlated with the four kinds of antioxidant activity indexes(p <0.01). The essential antioxidant substances in Elaeagnus mollis Diels seed oil were γ-tocopherol, δ-tocopherol and lupeol.
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