刘超.高脂饮食诱导高脂血症大鼠及不同器官病理变化[J].中国油脂,2020,45(9):81~84.[LIU Chao.Hyperlipidemia induced by high-fat diet in rats and pathological changes in different organs[J].China Oils and Fats,2020,45(9):81~84.]
高脂饮食诱导高脂血症大鼠及不同器官病理变化
Hyperlipidemia induced by high-fat diet in rats and pathological changes in different organs
  
DOI:
中文关键词:  高脂饮食;高脂血症;大鼠;PD-1;免疫性炎症
英文关键词:high-fat diet; hyperlipidemia; rat; PD-1; immune inflammation
基金项目:山东省中医药科技发展计划项目(2019-0455);济宁医学院大学生科研项目(JYXS2017KJ022);济宁医学院校级教育科学研究项目(18066);济宁医学院青年教师科研扶持基金(JY2017KJ046)
作者单位
刘超 济宁医学院 药学院, 山东 日照 276826 
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中文摘要:
      通过高脂饮食建立高脂血症大鼠模型,观察高脂血症大鼠体内程序性死亡受体1(PD-1)的表达变化。将40只雄性SD大鼠分为2组,分别饲喂普通饲料和高脂饲料12周,计算大鼠的肝脏、脾脏和胰腺指数,测定大鼠全血血糖,检测大鼠血清中甘油三酯(TG)、总胆固醇(T-CHO)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇( LDL-C)的含量,通过HE染色检测大鼠肝脏的病理变化,同时采用RT-qPCR检测PD-1 mRNA在大鼠肝脏、脾脏和胰腺组织内的表达变化。结果表明:高脂饲料组大鼠的肝脏、脾脏和胰腺指数显著高于普通饲料组;高脂饲料组大鼠的血糖显著升高;高脂饲料组大鼠血清中的TG、T-CHO、LDL-C的含量均显著高于普通饲料组,LDL-C/HDL-C的比值也显著高于普通饲料组,高脂血症大鼠造模成功。HE染色显示高脂饲料组大鼠肝脏组织内均出现明显脂肪空泡。与普通饲料组相比,高脂饲料组大鼠肝脏、脾脏和胰腺组织内PD-1 mRNA的表达均显著降低,提示PD-1可能参与了高脂血症的发生和发展。
英文摘要:
      In order to establish hyperlipidemia rats model by high-fat diet and observe expression of PD-1 in hyperlipidemia rats, forty male SD rats were divided into two groups of high-fat diet group and normal diet group and fed for twelve weeks with normal feed and high-fat feed, respectively. The liver index, spleen index and pancreas index were calculated. The blood glucose and serum triglyceride (TG), total cholesterol (T-CHO) and high density lipoprotein (HDL-C) and low density lipoprotein (LDL-C) concentrations were determined. HE staining was used to observe the pathological changes of rat liver, and PD-1 mRNA expressions in rat liver, spleen and pancreas were detected by RT-qPCR. The results showed that the liver index, spleen index and pancreas index in the high-fat diet group were significantly higher than those in the normal diet group. The blood glucose in the high-fat diet group was significantly increased, the serum levels of TG, T-CHO, HDL-C and LDL-C in the high-fat diet group were higher than those in the normal diet group, and the ratio of LDL-C to HDL-C was also significantly higher than that in the normal diet group. HE staining showed that there were obvious fat vacuoles in the liver tissue of rats fed with high-fat diet. The expressions of PD-1 mRNA in liver, spleen and pancreas tissue of rats fed with high-fat diet were significantly lower than that of rats fed with nomal diet, indicating that PD-1 might participate in the occurrence and development of hyperlipidemia.
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