周松峰,房磊.琉璃苣籽油的萃取及对力竭运动大鼠骨骼肌抗氧化酶活性的影响[J].中国油脂,2022,47(12):.[ZHOU Songfeng, FANG Lei.Extraction of borage seed oil and its effect on antioxidant enzyme activity of skeletal muscle in exhaustive exercise rats[J].China Oils and Fats,2022,47(12):.]
琉璃苣籽油的萃取及对力竭运动大鼠骨骼肌抗氧化酶活性的影响
Extraction of borage seed oil and its effect on antioxidant enzyme activity of skeletal muscle in exhaustive exercise rats
  
DOI:
中文关键词:  琉璃苣籽油  力竭运动  骨骼肌  抗氧化酶
英文关键词:borage seed oil  exhaustive exercise  skeletal muscle  antioxidant enzyme
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作者单位
周松峰,房磊 吉林农业科技学院长春130000 
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中文摘要:
      为了促进琉璃苣籽油的开发利用,以琉璃苣籽为原料,采用亚临界丁烷萃取琉璃苣籽油,通过单因素实验研究液料比、萃取温度、萃取时间对琉璃苣籽油萃取率的影响,通过响应面实验对琉璃苣籽油萃取工艺条件进行优化,并对最佳条件下萃取的琉璃苣籽油脂肪酸组成进行分析。将SD大鼠平均分成5组,对照组不进行训练,剩余4组进行力竭运动训练,低、中、高剂量组每日灌胃10、15、20 mg/g琉璃苣籽油,力竭运动组每日灌胃15 mg/g蒸馏水,对照组正常饮水,实验结束后测定大鼠骨骼肌中抗氧化酶活性,考察琉璃苣籽油对力竭运动大鼠骨骼肌抗氧化酶活性的影响。结果表明:琉璃苣籽油最佳萃取工艺条件为液料比6.2∶1、萃取温度41℃、萃取时间53 min,在该条件下琉璃苣籽油萃取率为35.32%;琉璃苣籽油中不饱和脂肪酸含量为85.51%;琉璃苣籽油可以恢复骨骼肌中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)的活性,降低骨骼肌中丙二醛(MDA)含量。琉璃苣籽油可以提高力竭运动大鼠骨骼肌抗氧化酶活性,提高机体清除自由基的能力,减少对机体细胞的损伤。
英文摘要:
      In order to promote the development and utilization of borage seed oil, taking borage seed as raw material, the borage seed oil was extracted with the subcritical butane extraction method. The effects of liquid-material ratio, extraction temperature and extraction time on the extraction rate of borage seed oil were studied by single factor experiment. The extraction conditions of borage seed oil were optimized by response surface methodology. The fatty acid composition of the borage seed oil was analyzed. In addition, SD rats were divided into five groups on average. The control group did not receive training, and the remaining four groups received exhaustive exercise training. The low, medium and high dose groups were gavaged with 10, 15 mg/g and 20 mg/g borage seed oil every day, the exhaustive exercise group was gavaged with 15 mg/g distilled water every day, and the control group drank water normally. After the experiment, the activity of antioxidant enzymes in the skeletal muscle of the rats was determined to investigate the effect of borage seed oil on the activity of antioxidant enzymes in the skeletal muscle of exhausted exercise rats. The results showed that the optimum extraction conditions of borage seed oil were as follows: liquid-material ratio 6.2∶1, extraction temperature 41℃, extraction time 53 min. Under these conditions, the extraction rate of borage seed oil was 35.32%. The content of unsaturated fatty acids in borage seed oil was 85.51%. The borage seed oil could restore the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in skeletal muscle, and reduce the content of malondialdehyde (MDA) in skeletal muscle. Borage seed oil can increase the activity of antioxidant enzymes in skeletal muscle of exhausted exercise rats, improve the body′s ability to scavenge free radicals and reduce the damage to body cells.
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