| 陈玲1，张立伟1,2，雷芬芬1,2，郑竟成1，何东平1,2.乳清分离蛋白-黄原胶复合乳化剂制备南瓜籽油O/W型乳液及其稳定性[J].中国油脂,2022,47(12):.[CHEN Ling1, ZHANG Liwei1,2, LEI Fenfen1,2, ZHENG Jingcheng1, HE Dongping1,2.Preparation of pumpkin seed oil O/W emulsion by whey protein isolate-xanthan gum compound emulsifier and its stability[J].China Oils and Fats,2022,47(12):.] |
| 乳清分离蛋白-黄原胶复合乳化剂制备南瓜籽油O/W型乳液及其稳定性 |
| Preparation of pumpkin seed oil O/W emulsion by whey protein isolate-xanthan gum compound emulsifier and its stability |
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| DOI： |
| 中文关键词: 南瓜籽油 乳清分离蛋白 黄原胶 O/W型乳液 稳定性 |
| 英文关键词:pumpkin seed oil whey protein isolate xanthan gum oil-in-water emulsion stability |
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| 中文摘要: |
| 为提高南瓜籽油（PSO）的稳定性，以及提高由单一乳清分离蛋白（WPI）作为乳化剂制备的水包油（O/W）型乳液的稳定性，制备了黄原胶（XG）与乳清分离蛋白协同稳定的南瓜籽油O/W型乳液，探究了黄原胶添加量及添加顺序对乳液性质及其稳定性的影响。结果表明：黄原胶质量浓度为2.0 mg/mL 时，乳液平均粒径最小，为（10.53±0.06）μm，而ζ-电位绝对值最大，为（37.92±0.61）mV，乳液稳定性最好；黄原胶添加顺序不同，乳液稳定性有所差别，其中乳液WPI-PSO-XG（乳清分离蛋白与南瓜籽油乳化得粗乳液，再加黄原胶二次分散得到的乳液）的物理和化学稳定性最好；加速氧化实验显示，乳液的过氧化值(POV)及硫代巴比妥酸反应物（TBARS）值均低于南瓜籽油，其中乳液WPI-PSO-XG的POV和TBARS值最低，与南瓜籽油相比，分别降低了16.13 mmol/kg和17.63 μmol/L，表现出良好的氧化稳定性。说明南瓜籽油与乳清分离蛋白制备成初乳液，再加入黄原胶，可使乳液稳定性提高。 |
| 英文摘要: |
| In order to improve the stability of pumpkin seed oil (PSO) and oil-in-water (O/W) emulsion prepared with whey protein isolate (WPI) as an single emulsifier, an O/W emulsion of pumpkin seed oil stabilized with xanthan gum (XG) and WPI was prepared, and the effects of the addition amount and order of XG on the properties and stability of the emulsion were studied. The results showed that when the mass concentration of XG was 2.0 mg/mL, the mean particle size of the emulsion was the smallest at (10.53±0.06)μm and the absolute value of the Zeta potential was the largest at (37.92±0.61 )mV, and the emulsion showed the best stability. The stability of the emulsion varied with the addition order of XG. The physical and chemical stability of the WPI-PSO-XG emulsion (prepared by sequential dispersion of a mixture of WPI and PSO, followed by addition of XG) was the best. The accelerated oxidation experiments showed that the peroxide value (POV) and thiobarbituric acid reactant (TBARS) value of emulsion were lower than those of PSO. Among them, the POV and TBARS value of the WPI-PSO-XG emulsion were the lowest, and compared with PSO, it was reduced by 16.13 mmol/kg and 17.63 μmol/L, respectively, showing good oxidation stability. Adding XG to the primary emulsion prepared with PSO and WPI can improve the stability of the emulsion. |
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