李顺发1,邓雨冰1,胡醇1,王良俊2,张清1.葡聚糖接枝大豆分离蛋白Pickering乳液的 制备与性质研究[J].中国油脂,2025,50(12):.[LI Shunfa1, DENG Yubing1, HU Chun1, WANG Liangjun2, ZHANG Qing1.Preparation and properties of dextran grafted soybean protein isolate-based Pickering emulsions[J].China Oils and Fats,2025,50(12):.]
葡聚糖接枝大豆分离蛋白Pickering乳液的 制备与性质研究
Preparation and properties of dextran grafted soybean protein isolate-based Pickering emulsions
  
DOI:10.19902/j.cnki.zgyz.1003-7969.240727
中文关键词:  Pickering乳液  乳液稳定性  乳液性质  大豆分离蛋白  大豆分离蛋白-葡聚糖共轭物
英文关键词:Pickering emulsions  emulsion stability  emulsion property  soybean protein isolate  soybean protein isolate-dextran conjugate
基金项目:四川天府新区眉山管委员会科技合作项目(2322339004);雅安市-四川农业大学市校合作项目(23ZDYF0003)
作者单位
李顺发1,邓雨冰1,胡醇1,王良俊2,张清1 1.四川农业大学 食品学院四川 雅安 625014 2.眉山天府新区乡村振兴发展服务中心四川 眉山 620000 
Author NameAffiliation
LI Shunfa1, DENG Yubing1, HU Chun1, WANG Liangjun2, ZHANG Qing1 1.College of Food Science, Sichuan Agricultural University, Ya′an 625014, Sichuan, China
2.Rural Revitalization and Development Service Center of Meishan Tianfu New District, Meishan 620000, Sichuan, China 
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中文摘要:
      旨在为脂溶性生物活性物质的包埋递送提供参考,以大豆分离蛋白(SPI)-葡聚糖共轭物(SDC)为乳化剂和稳定剂,采用两步法运用高压微射流制备Pickering乳液(SDC-PEs),通过单因素实验研究了均质压力、均质次数、SDC添加量对乳液粒径和多分散指数(PDI)的影响,并采用响应面实验对SDC-PEs的制备条件进行了优化。另外,对最佳工艺条件下制备的SDC-PEs性质进行了分析。结果表明:SDC-PEs的最佳制备工艺条件为均质压力140 MPa 、均质次数6次、SDC添加量2.13%、油相体积分数10%,在此条件下SDC-PEs的平均粒径为210.33 nm,PDI小于0.20;SDC-PEs表现出良好的pH稳定性、抗离子强度稳定性、温度稳定性和储存稳定性,且优于以SPI稳定的Pickering乳液(SPI-PEs)。综上,通过添加SDC可以制备出稳定性良好的Pickering乳液。
英文摘要:
      To provide a reference for the encapsulation and delivery of lipophilic bioactive substances, Pickering emulsions (SDC-PEs)was prepared by using a two-step method of high-pressure microfluidization process with soybean protein isolate(SPI)-dextran conjugate(SDC) as emulsifier and stabilizer. Single-factor experiments were conducted to investigate the effects of homogenization pressure, homogenization times, and SDC dosage on emulsion particle size and polydispersity index (PDI). The preparation conditions of SDC-PEs were further optimized using response surface methodology. Additionally, the properties of SDC-PEs prepared under the optimal conditions were analyzed. The results showed that the optimal preparation parameters of SDC-PEs were as follows: homogenization pressure 140 MPa, homogenization times 6, SDC dosage 2.13%, and volume fraction of oil phase 10%. Under these conditions, SDC-PEs exhibited an average particle size of 210.33 nm with a PDI below 020. The SDC-PEs showed excellent stability against pH, ionic strength, temperature, and storage, outperforming SPI-stabilized Pickering emulsions (SPI-PEs). In conclusion, Pickering emulsions with good stability can be prepared by adding SDC.
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