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| Influences of three typical unsaturated fatty acids on mechanical barrier function of Caco-2 intestinal epithelial cells |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240682 |
| KeyWord:unsaturated fatty acids intestinal mechanical barrier Caco-2 cell model nutritional attributes |
| FundProject:“十四五”国家重点研发计划（2023YFD2100400）；国家自然科学基金青年基金（32201936）；江苏省自然科学基金青年基金（BK20221076） |
| Author Name | Affiliation | | DU Shumeng1, LI Rui1, LIU Yuanfa1,2, YANG Wei1,2, YE Zhan1,2 | 1.School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China
2.State Key Laboratory of Food Science and Resources, Wuxi 214122, Jiangsu, China |
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| Abstract: |
| In order to provide a theoretical basis for optimizing dietary fat intake structure and preventing related chronic metabolic diseases, by constructing a Caco-2 intestinal epithelial cell mechanical barrier model, the effects of different fatty acids (oleic acid (OA), linoleic acid (LA), and α-linolenic acid (ALA)) on the integrity and permeability of the Caco-2 cell model were investigated, as well as their regulatory effects on the expression levels of genes and key proteins related to the mechanical barrier of Caco-2 cells. The results showed that under the conditions of fatty acid concentration 300 μmol/L and intervention time 24 h, LA significantly reduced the transmembrane resistance value and alkaline phosphatase specific activity, increased the apparent osmotic coefficient and nitric oxide content, while OA and ALA had no significant effect on the above indicators. LA significantly downregulated the expression levels of the tight junction protein 1 (ZO1) gene and the occludin (OCLN) gene, but had no significant effect on the expression of the claudin 2 (CLDN2) gene. ALA significantly increased the expression level of the ZO1 gene and inhibited the expression level of the CLDN2 gene, while OA had no significant effect on the expression levels of the ZO1, OCLN, and CLDN2 genes. The three fatty acids had no significant effects on the expression of barrier adhesion junction protein and desmosome protein-related genes. LA significantly reduced the expression levels of tight junction protein 1(ZO-1) and Occludin, ALA significantly reduced the expression level of Claudin-2, and OA had no significant effect on the expression levels of tight junction proteins. In conclusion, the three typical unsaturated fatty acids have different effects on the barrier function of Caco-2 cells. LA can reduce the integrity of the intestinal barrier and increase its permeability by lowering the expression level of tight junction proteins, ALA promotes the tightness of the intestinal barrier, while OA has no obvious influence. |
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