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| Composition and positional distribution of fatty acids in triacylglycerols and glycerophospholipids from the viscera oil of four sources squids |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240626 |
| KeyWord:squid viscera fish oil fatty acid positional distribution |
| FundProject:江西省自然科学基金青年基金项目（20232BAB215060）；国家自然科学基金项目（32060557）；2023年度浙江省博士后科研项目择优资助（319030） |
| Author Name | Affiliation | | CUI Ning1, LI Jinlin1, HU Mingming1, TU Zongcai1,2,
CHEN Yunyun3, PENG Bin1 | 1.National R&D Branch Center for Conventional Freshwater Fish Processing, College of Life Science, Jiangxi
Normal University, Nanchang 330022, China
2.State Key Laboratory of Food Science and Resources,
Nanchang University, Nanchang 330047, China
3.China Aquatic Products Zhoushan Marine Fisheries
Corporation, Zhoushan 316000, Zhejiang, China |
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| Abstract: |
| To explore the nutritional value of squid visceral lipids, oils were extracted from the viscera of four sources squids(Argentine, North Pacific, Equatorial and Peruvian) using ultrasound-assisted solvent extraction. The fatty acid composition of triacylglycerols and glycerophospholipids in squid visceral oils, as well as their positional distribution on the glycerol backbone, were analyzed by solid-phase extraction (SPE), regioselective enzymatic hydrolysis, and gas chromatography-mass spectrometry (GC-MS). The results showed that a total of 42 fatty acids were identified in triacylglycerols from the viscera oil of four squids, including 18 saturated fatty acids (SFA), 9 monounsaturated fatty acids (MUFA), and 15 polyunsaturated fatty acids (PUFA). The relative contents of SFA, MUFA, and PUFA in triacylglycerols were 31.79%-33.24%, 32.99%-3502%, and 33.19%-33.90%, respectively. The triacylglycerols from Peruvian, and Equatorial squid viscera oil showed the highest SFA contents (33.24% and 33.11%, respectively), which were significantly higher than those from Argentine and North Pacific squids (p<0.05). There was no significant difference in MUFA content of triacylglycerols of viscera oil between Equatorial and Peruvian squids(p>0.05), but their levels were significantly lower than those from Argentine and North Pacific squids (p<0.05). Regarding PUFA, no significant differences were found among Equatorial, Argentine, and Peruvian squids (p>005), but their levels were significantly higher than that in North Pacific squid (p<0.05). MUFA were predominantly esterified at the Sn-2 position of triacylglycerols (39.78%-41.98%), while SFA and PUFA showed preferential localization at the Sn-1,3 positions, accounting for 32.84%-34.15% and 35.62%-36.34% of total fatty acids, respectively. A total of 83 fatty acids were identified in glycerophospholipids, comprising 21 SFA, 19 MUFA, and 43 PUFA. The relative contents of these fatty acid classes in glycerophospholipids followed the hierarchical order: PUFA>SFA>MUFA. The contents of PUFA in visceral glycerophospholipids in Argentine and North Pacific squids were significantly higher than those in Equatorial and Peruvian squids (p<0.05). The fatty acids at the Sn-1 position of the glycerophospholipids were primarily SFA and MUFA, accounting for 40.60%-40.91% and 25.10%-25.28%, respectively. In contrast, the Sn-2 position was predominantly occupied by PUFA (69.95%-70.10%). In conclusion, triacylglycerols and glycerophospholipids of squid viscera oils from four different sources differ significantly in fatty acid composition, and the acylation positions of different fatty acids on the glycerol backbone are selective. |
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