| 姚雨玲1，2, 郭娟2，强奇2，庞志强2，李绍朋3,田波2.印度血桐（Macaranga indica）种子中与神经酸累积相关的内生真菌研究[J].中国油脂,2026,51(7):.[YAO Yuling1，2, GUO Juan2, QIANG Qi2, PANG Zhiqiang2， LI Shaopeng3, TIAN Bo2.Endophytic fungi producing nervonic acid from Macaranga indica seeds[J].China Oils and Fats,2026,51(7):.] |
| 印度血桐（Macaranga indica）种子中与神经酸累积相关的内生真菌研究 |
| Endophytic fungi producing nervonic acid from Macaranga indica seeds |
|
投稿时间：2025-04-18 修订日期：2025-12-22 录用日期：2025-05-21
出版日期：2026-07-20
|
| DOI：10.19902/j.cnki.zgyz.1003-7969.250200 |
| 中文关键词: 内生菌 分离鉴定 脂肪酸 神经酸 印度血桐 |
| 英文关键词:endophyte isolation and identification fatty acid nervonic acid Macaranga indica |
| 基金项目:云南省中青年学术和技术带头人后备人才项目（202105AC160083） |
|
| Author Name | Affiliation | | YAO Yuling1，2, GUO Juan2, QIANG Qi2, PANG Zhiqiang2， LI Shaopeng3, TIAN Bo2 | 1.College of Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500,
China
2.Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Xishuangbanna
666303,Yunnan,China
3.Pu′er Tea College, West Yunnan University of Applied Sciences,
Pu′er 671006,Yunnan,China |
|
| 摘要点击次数: 436 |
| 全文下载次数: 3 |
| 中文摘要: |
| 为探究印度血桐作为生产神经酸微生物资源的潜力，采用不同培养基对印度血桐种仁及种仁油进行培养，将分离纯化的内生菌通过16S rRNA和ITS扩增子测序进行分子鉴定。对纯化获得的内生真菌进行脂肪酸组成测定，进一步在培养基中添加芥酸作为底物培养后检测其脂肪酸组成。结果表明：印度血桐种子中分离到细菌共153株，分属于17个属，26种，真菌共168株，分属于26个属，47种；内生真菌的脂肪酸组成以棕榈酸、油酸和亚油酸为主，未检测到神经酸，但有6个内生真菌检测到芥酸（神经酸前体物质），其中，添加芥酸作为底物进行培养，内生真菌亮白曲霉（Aspergillus candidus）能够产生1.32%的神经酸。综上，印度血桐种子中内生真菌可能有促进神经酸累积的作用，因此其具有作为微生物资源生产神经酸的潜力。 |
| 英文摘要: |
| In order to explore the potential of the Macaranga indica as a microbial resource for nervonic acid production, different culture media were used to cultivate the seeds and seed oil of Macaranga indica, and the isolated and purified endophytes were identified by 16S rRNA and ITS amplicon sequencing. The fatty acid composition of the purified endophytic fungi was determined, and further erucic acid was added as a substrate to the culture medium to detect their fatty acid composition after cultivation. The results showed that a total of 153 strains of bacteria belonging to 17 genera and 26 species were isolated from the seeds of the Macaranga indica. There were a total of 168 fungi, belonging to 26 genera and 47 species. The fatty acid composition of endophytic fungi was mainly composed of palmitic acid, oleic acid, and linoleic acid, nervonic acid was not detected, but erucic acid (a precursor of nervonic acid) was detected from 6 endophytic fungi. Among them, when erucic acid was added as a substrate for cultivation, the Aspergillus candidus was able to produce 132% of nervonic acid. In summary, endophytic fungi from Macaranga indica seeds may facilitate the accumulation of nervonic acid, indicating the potential of these seeds as a microbial resource for nervonic acid production. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
