徐洪文.高效液相色谱法测定食用植物油中6种真菌毒素[J].中国油脂,2020,45(11):77~83.[XU Hongwen.Detection of six mycotoxins in edible vegetable oil by high performance[J].China Oils and Fats,2020,45(11):77~83.]
高效液相色谱法测定食用植物油中6种真菌毒素
Detection of six mycotoxins in edible vegetable oil by high performance
  
DOI:10.12166/j.zgyz.1003-7969/2020.11.016
中文关键词:  真菌毒素;高效液相色谱法;液液萃取技术;食用植物油
英文关键词:mycotoxins; high performance liquid chromatography; liquid-liquid extraction technology; edible vegetable oil
基金项目:“十三五”国家重点研发计划(2018YFC1604206);国家自然科学基金(31772069)
作者单位
徐洪文 江南大学 食品科学与技术国家重点实验室,食品安全与营养协同创新中心,江苏 无锡 214122 
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中文摘要:
      建立了基于分散液液微萃取技术(DLLME)的高效液相色谱法同时测定食用植物油中6种真菌毒素的方法。样品经石油醚脱脂,乙腈-水-乙酸(体积比84∶ 15∶ 1)超声提取,CHCl3为萃取剂液液萃取净化及柱前衍生化后,以Agilent XDB-C18色谱柱(4.6 mm×150 mm, 3.5 μm)分离,梯度洗脱,经荧光检测器检测。结果表明:食用植物油中6种真菌毒素的线性关系良好,相关系数均大于0.999,方法检出限为0.2~0.5 μg/kg,样品的平均加标回收率为75.88%~105.25%,相对标准偏差为0.5%~9.5%。该方法可用于食用植物油中黄曲霉毒素(AFB1、AFB2、AFG1、AFG2)以及赭曲霉毒素(OTA、OTB)6种真菌毒素的同时检测。
英文摘要:
      A method for simultaneous determination of six mycotoxins in edible vegetable oil by high performance liquid chromatography(HPLC) based on dispersion liquid-liquid microextraction technology (DLLME) was established. The sample was defatted with petroleum ether, ultrasound-extracted with acetonitrile-water-acetic acid (volume ratio 84∶ 15∶ 1), purified by liquid-liquid extraction with CHCl3 as extractant and pre-column derived.Then, it was separated by Agilent XDB-C18 chromatographic column (4.6 mm×150 mm, 3.5 μm) with gradient elution, and detected by fluorescence detector.The results showed that the linear relationships of the six mycotoxins in edible vegetable oil were good and the correlation coefficients were all more than 0.999.The detection limit of the method was 0.2-0.5 μg/kg.The average recovery of the samples was 75.88%-105.25%, and the relative standard deviation was 0.5%-9.5%.This method could be used for the simultaneous detection of six mycotoxins including aflatoxins (AFB1,AFB2,AFG1,AFG2) and ochratoxin (OTA, OTB) in edible vegetable oil.
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