殷金莲 靳 毓.酶解法制备榛子粕ACE抑制肽工艺研究[J].中国油脂,2020,45(1):68~72.[YIN Jinlian JIN Yu.Preparation of ACE inhibitory peptide from hazelnut meal by enzymatic hydrolysis[J].China Oils and Fats,2020,45(1):68~72.]
酶解法制备榛子粕ACE抑制肽工艺研究
Preparation of ACE inhibitory peptide from hazelnut meal by enzymatic hydrolysis
  
DOI:10.12166/j.zgyz.1003-7969/2020.01.015
中文关键词:  榛子粕;ACE抑制肽;酶解;中性蛋白酶;ACE抑制率
英文关键词:hazelnut meal; ACE inhibitory peptide; enzymatic hydrolysis; neutral protease; ACE inhibition rate
基金项目:
作者单位
殷金莲 靳 毓 1.朔州职业技术学院 生物工程系,山西 朔州 036002; 2.西北农林科技大学 食品科学与工程学院,陕西 杨凌 712100 
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中文摘要:
      以榛子粕为原料,利用中性蛋白酶酶解制备ACE抑制肽。以酶解产物的ACE抑制率为指标,采用单因素试验分别考察底物质量分数、酶用量、酶解温度、pH和酶解时间的影响。在单因素试验基础上设计响应面Box-Behnken 中心组合试验对酶解条件进行优化。结果表明:榛子粕最佳酶解工艺条件为底物质量分数5%、酶用量0.3%、酶解温度40 ℃、pH 7.5、酶解时间1.5 h,在此条件下榛子粕酶解产物的ACE抑制率达到91.76%。
英文摘要:
      The hazelnut meal was hydrolyzed by neutral protease to prepare ACE inhibitory peptide. Taking the ACE inhibition rate of the enzymatic hydrolysates as an index, single factor experiment was carried out to investigate the effects of substrate mass fraction, enzyme dosage, enzymatic hydrolysis temperature, pH and enzymatic hydrolysis time. On the basis of single factor experiment, response surface Box-Behnken central combination experiment was designed to optimize the enzymatic hydrolysis conditions. The results showed that the optimal enzymatic hydrolysis conditions were obtained as follows: substrate mass fraction 5%, enzyme dosage 0.3%, enzymatic hydrolysis temperature 40 ℃, pH 7.5 and enzymatic hydrolysis time 1.5 h. Under these conditions, the ACE inhibition rate of enzymatic hydrolysates could reach 91.76%.
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