| 董杨帅1，徐思渊1，于博安1，李艳艳1，陈小红2，李红娟1，于景华1，李洪波1.高压均质处理下β-乳球蛋白/亚油酸复合物的
结构变化及其制备工艺优化[J].中国油脂,2026,51(5):.[DONG Yangshuai1, XU Siyuan1, YU Bo′an1, LI Yanyan1, CHEN Xiaohong2,
LI Hongjuan1, YU Jinghua1, LI Hongbo1.Structural changes and process optimization of β-lactoglobulin/linoleic acid complexes under high-pressure homogenization[J].China Oils and Fats,2026,51(5):.] |
| Structural changes and process optimization of β-lactoglobulin/linoleic acid complexes under high-pressure homogenization |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.250147 |
| KeyWord:β-lactoglobulin linoleic acid high-pressure homogenization structural characteristics |
| FundProject:天津市科技计划项目（23JCYBJC01160，23YDTTPJC00340） |
| Author Name | Affiliation | | DONG Yangshuai1, XU Siyuan1, YU Bo′an1, LI Yanyan1, CHEN Xiaohong2,
LI Hongjuan1, YU Jinghua1, LI Hongbo1 | 1.School of Food Science and Engineering, Tianjin University of Science and Technology,
Tianjin 300457, China
2.Tianjin Haihe Dairy Co. , Ltd. , Tianjin 300457, China |
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| Abstract: |
| To provide a reference for the development of nutritionally enhanced foods, with β-lactoglobulin (β-LG)/linoleic acid complexes prepared under different homogenization pressures as the material, the effect of homogenization pressure on the structural characteristics of β-LG/linoleic acid complexes were studied using spectroscopic techniques. Based on preliminary experiments, response surface methodology was employed to optimize the preparation conditions of the complexes, with the molar ratio of β-LG to linoleic acid, reaction time, and homogenization pressure as independent variables, and the particle size of the complexes as the response value. The results showed that as the homogenization pressure increased from 0 MPa to 120 MPa, the intrinsic fluorescence intensity of the complexes first decreased and then increased, while the UV absorption and surface hydrophobicity first increased and then decreased. Similarly, the α-helix content initially decreased and then increased, whereas the β-sheet content showed the opposite trend. The optimal preparation conditions were determined to be a reaction time of 10 min, homogenization pressure of 80 MPa, and a β-LG to linoleic acid molar ratio of 1∶ 1, yielding a complex particle size of 210.26 nm. In conclusion, high-pressure homogenization significantly alters the structure of β-LG/linoleic acid complexes. |
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