| 浦若颖1,2，董仕豪1,2，游小杰2，黄雅芳2，韩龙1,2，范方宇1,2，郭磊1,2.超声辅助酶法提取花生粕可溶性膳食
纤维及其生物活性研究[J].中国油脂,2026,51(5):.[PU Ruoying1,2, DONG Shihao1,2, YOU Xiaojie2, HUANG Yafang2,
HAN Long1,2, FAN Fangyu1,2, GUO Lei1,2.Ultrasound-assisted enzymatic extraction of soluble dietary fiber from peanut meal and its bioactivity[J].China Oils and Fats,2026,51(5):.] |
| 超声辅助酶法提取花生粕可溶性膳食
纤维及其生物活性研究 |
| Ultrasound-assisted enzymatic extraction of soluble dietary fiber from peanut meal and its bioactivity |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.250425 |
| 中文关键词: 花生粕 可溶性膳食纤维 抗氧化活性 α-淀粉酶抑制活性 α-葡萄糖苷酶抑制活性 |
| 英文关键词:peanut meal soluble dietary fiber antioxidant activity α-amylase inhibitory activity α-glucosidase inhibitory activity |
| 基金项目:中央引导地方科技发展资金自由探索类基础研究项目（202407AA110007） |
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| Author Name | Affiliation | | PU Ruoying1,2, DONG Shihao1,2, YOU Xiaojie2, HUANG Yafang2,
HAN Long1,2, FAN Fangyu1,2, GUO Lei1,2 | 1.Forest Resources Exploitation and Utilization Engineering Research Center for Grand Health of Yunnan
Provincial Universities, Southwest Forestry University, Kunming 650224, China
2.College of Biological
Sciences and Food Engineering, Southwest Forestry University, Kunming 650224, China |
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| 中文摘要: |
| 旨在为花生粕的高值化利用及功能性食品原料的开发提供参考，以花生为原料，经压榨、溶剂脱脂后获得花生粕，采用超声辅助酶法（超声预处理后进行酶解）从花生粕中提取可溶性膳食纤维（SDF），通过单因素试验与响应面试验确定了SDF的最优提取条件，并通过体外试验研究了SDF的抗氧化活性与降血糖活性。结果表明：SDF的最优提取条件为料液比1∶ 30、超声时间28 min、超声功率250 W、纤维素酶添加量1.9%（以花生粕质量计）、酶解温度50 ℃、酶解pH 5.0、酶解时间1.5 h，在此条件下SDF得率达到13.87%；花生粕SDF对DPPH自由基、ABTS+自由基及羟自由基的半数清除浓度（IC50 值）分别为2.907、2.886 mg/mL和0.057 mg/mL；SDF对α-葡萄糖苷酶和α-淀粉酶的半数抑制浓度（IC50 值)分别为0.051 mg/mL和0.205 mg/mL。综上，超声辅助酶法可有效提取花生粕中SDF，且所提取的SDF具有良好的体外抗氧化活性和降血糖活性。 |
| 英文摘要: |
| To provide a reference for the high-value utilization of peanut meal and the development of functional food ingredients, with peanut as raw material, peanut meal was obtained by pressing and solvent defatting. Soluble dietary fiber(SDF) was extracted from peanut meal using an ultrasound-assisted enzymatic method (ultrasonic pretreatment followed by enzymatic hydrolysis). The optimal extraction conditions for SDF were determined by single-factor test and response surface methodology, and the antioxidant activity and hypoglycemic activity of SDF were investigated through in vitro experiments. The results showed that the optimal extraction conditions for SDF were as follows: solid-liquid ratio 1∶ 30, ultrasonic time 28 min, ultrasonic power 250 W, cellulase dosage 1.9% (based on the mass of peanut meal), enzymatic hydrolysis temperature 50 ℃, enzymatic hydrolysis pH 5.0, and enzymatic hydrolysis time 1.5 h. Under these conditions, the SDF yield reached 13.87%. The half-clearing concentrations (IC50 values) of peanut meal SDF against DPPH radical, ABTS+ radical, and hydroxyl radical were 2.907, 2.886 mg/mL, and 0.057 mg/mL, respectively. The half-inhibitory concentrations (IC50 values) of SDF against α-glucosidase and α-amylase were 0.051 mg/mL and 0.205 mg/mL, respectively. In conclusion, ultrasound-assisted enzymatic extraction can effectively extract SDF from peanut meal, and the extracted SDF exhibits good in vitro antioxidant activity and hypoglycemic activity. |
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