| .乳脂中1,3-甘油二酯检测方法的研究[J].中国油脂,2011,(7):.[.Determination of 1,3 - diacylglycerol in milk fat[J].China Oils and Fats,2011,(7):.] |
| 乳脂中1,3-甘油二酯检测方法的研究 |
| Determination of 1,3 - diacylglycerol in milk fat |
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| 中文关键词: 乳脂 1,3-甘油二酯 薄层色谱法 甘油氧化酶法 |
| 英文关键词:milk fat 1 3-diacylglycerol TLC GPO-PAP |
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| 中文摘要: |
| 建立一种乳脂中1,3-甘油二酯(1,3-DG)定性定量的检测方法.通过薄层色谱法(TLC)对乳脂中1,3-甘油二酯进行分离和定性,再用甘油氧化酶法进行定量检测.研究结果显示:薄层色谱法能有效分离出1,3-甘油二酯;定量方法RSD小于等于3.78%,回收率在86.73%～101.83%之间.该方法能准确、快速、灵敏地分离检测乳脂中的1,3-甘油二酯. |
| 英文摘要: |
| A qualitative and quantitative determination of the 1,3-diacylglycerol(1,3-DG) in milk fat was established.The 1,3-DG was effectively separated and qualitatively identified by TLC,and then was quantitatively analyzed by GPO-PAP.The RSD of the method was no more than 3.78% and the recovery was 86.73%-101.83%.The method was accurate,precise,rapid and suitable for the analysis of 1,3-DG in milk fat. |
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