| 史俊文,刘凤芝.免疫亲和柱净化-高效液相色谱-三重串联四级杆质谱法测定食用植物油中的黄曲霉毒素B1[J].中国油脂,2014,39(5):.[SHI Junwen,LIU Fengzhi.Determination of aflatoxin B1 in edible vegetable oil by immunoaffinity column purification and HPLC-MS/MS[J].China Oils and Fats,2014,39(5):.] |
| 免疫亲和柱净化-高效液相色谱-三重串联四级杆质谱法测定食用植物油中的黄曲霉毒素B1 |
| Determination of aflatoxin B1 in edible vegetable oil by immunoaffinity column purification and HPLC-MS/MS |
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| DOI： |
| 中文关键词: 免疫亲和柱净化 高效液相色谱-三重串联四级杆质谱法 黄曲霉毒素B1 食用植物油 |
| 英文关键词:immunoaffinity column purification HPLC-MS/MS aflatoxin B1 edible vegetable oil |
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| 中文摘要: |
| 建立了免疫亲和柱净化-高效液相色谱-三重串联四级杆质谱法测定食用植物油中黄曲霉毒素B1的方法。采用70%甲醇水溶液提取食用植物油中黄曲霉毒素B1，提取液经过滤、免疫亲和柱净化后，上高效液相色谱-三重串联四级杆质谱仪进行测定。结果表明，黄曲霉毒素B1在0.2~10 ng/mL范围内方程线性关系良好，方法检出限（S/N=3）为0.05 μg/kg， 定量限（S/N=10）为0.2 μg/kg，平均加标回收率在83.0%~94.8%之间，相对标准偏差小于7.8%。 |
| 英文摘要: |
| Immunoaffinity column purification and HPLC-MS/MS method was established to determine aflatoxin B1 in edible vegetable oil. Aflatoxin B1 was extracted from edible vegetable oil by 70% of methanol,then the extract was filtered and purified by immunoaffinity column before being injected into HPLC-MS/MS to determine aflatoxin B1. The results showed that calibration curve presented good linear relationship when the mass concentration of aflatoxin B1 ranged from 0.2 ng/mL to 10 ng/mL;the limit of detection（S/N=3） and the limit of quantification（S/N=10） of the method were 0.05 μg/kg and 0.2 μg/kg respectively,the average recovery of standard addition was in the range of 83.0%-94.8%, and the relative standard deviation was less than 7.8%. |
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