| 邢 飞,朱靖博,丁 燕，等.载银氨基硅胶柱分离EPA和DHA的研究[J].中国油脂,2014,39(9):.[XING Fei,ZHU Jingbo,DING Yan，etc.Separation of EPA and DHA by Ag+ modified amino silica gel column[J].China Oils and Fats,2014,39(9):.] |
| 载银氨基硅胶柱分离EPA和DHA的研究 |
| Separation of EPA and DHA by Ag+ modified amino silica gel column |
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| DOI： |
| 中文关键词: EPA DHA 正相色谱 载银氨基硅胶 |
| 英文关键词:EPA DHA normal phase chromatography Ag+ modified amino silica gel |
| 基金项目: |
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| 中文摘要: |
| 利用负载银离子的氨基硅胶为固定相，制备4.6 mm×250 mm载银氨基硅胶柱，系统评价EPA和DHA在正相体系中的色谱保留行为，考察了载银氨基硅胶的粒径和流动相对EPA和DHA分离的影响。结果表明：5 μm粒径的载银氨基硅胶比10 μm粒径的分离度好；随着流动相中正己烷比例的增大，EPA和DHA分离度增大；当选用5 μm载银氨基硅胶、流动相为正己烷-异丁醇（体积比2 000∶1）、流速1 mL/min、紫外检测波长210 nm、进样量5 μL时，EPA和DHA的分离度最佳，分离度为2.03。该方法简单、快速、重现性好，可用于EPA和DHA的分离。 |
| 英文摘要: |
| The analysis column (4.6 mm×250 mm) for the separation of EPA and DHA was prepared with Ag+ modified amino silica gel as stationary phase. A systematic evaluation on retention behavior of EPA and DHA in normal phase chromatography was performed. The influences of Ag+ modified amino silica gel with different pore sizes and mobile phase on the separation of EPA and DHA were researched. The results showed that the separation degree of 5 μm Ag+ modified amino silica gel was better than that of 10 μm Ag+ modified amino silica gel; the separation degree of EPA and DHA increased with the proportion of n-hexane in mobile phase increasing; when the pore size of Ag+ modified amino silica gel was 5 μm, the volume ratio of n-hexane to isobutanol was 2 000∶1, flow rate was 1 mL/min, ultraviolet detection wavelength was 210 nm and the injection volume was 5 μL, the separation degree of EPA and DHA was the best and reached 2.03. The method had the items of simpleness, rapidness and high reproducibility, which was suitable for the separation of EPA and DHA. |
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