| 张云丰,汪立平.大孔树脂纯化茶皂素工艺研究[J].中国油脂,2014,39(11):.[ZHANG Yunfeng,WANG Liping.Purification of tea saponin by macroporous resin[J].China Oils and Fats,2014,39(11):.] |
| 大孔树脂纯化茶皂素工艺研究 |
| Purification of tea saponin by macroporous resin |
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| DOI： |
| 中文关键词: 茶皂素 大孔树脂 吸附 解吸 纯化 |
| 英文关键词:tea saponin macroporous resin adsorption desorption purification |
| 基金项目: |
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| 摘要点击次数: 1352 |
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| 中文摘要: |
| 采用大孔树脂对茶皂素粗品进行分离纯化。以茶皂素得率为指标，采用响应面法优化洗脱条件。通过考察不同极性的大孔树脂D-101、AB-8、S-8、X-5、HPD-100的吸附与解吸性能，从中筛选出具有代表性的S-8大孔树脂，并对其吸附和解吸能力进行综合分析。确定S-8大孔树脂纯化茶皂素最佳工艺条件为：S-8大孔树脂装柱量100 g，质量浓度为0.703 mg/mL的茶皂素溶液110 mL，上样流速3.03 mL/min，洗脱剂乙醇体积分数90%，乙醇流速3.03 mL/min，乙醇用量100 mL。在最佳条件下，对含量为47.38%的茶皂素粗品进行分离纯化，最终茶皂素得率为81.74%，回收率为78.29%，含量为85.36%。 |
| 英文摘要: |
| The crude tea saponin was separated and purified by macroporous resin. Using the yield of tea saponin as indicator, the elution conditions were optimized by response surface methodology. S-8 macroporous resin was screened out by studying the adsorption and desorption properties of macroporous resins D-101, AB-8, S-8, X-5 and HPD-100. The optimal adsorption conditions were obtained as follows: dosage of S-8 macroporous resin 100 g, mass concentration of tea sapoin solution 0.703 mg/mL, loading volume 110 mL and flow rate of sample 3.03 mL/min， volume fraction of ethanol (eluent)90%, flow rate 3.03 mL/min, dosage of ethanol 100 mL. After 47.38% of tea saponin was separated and purified under the optimal conditions, the yield, recovery rate and content of tea saponin were 81.74%, 78.29% and 85.36%,respectively. |
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