| 陈丽娟,卢佳思,张云华，等.HPLC测定棉籽粕中游离棉酚含量[J].中国油脂,2015,40(1):.[CHEN Lijuan,LU Jiasi,ZHANG Yunhua，etc.Determination of free gossypol in cottonseed meal by HPLC[J].China Oils and Fats,2015,40(1):.] |
| HPLC测定棉籽粕中游离棉酚含量 |
| Determination of free gossypol in cottonseed meal by HPLC |
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| DOI： |
| 中文关键词: 棉籽粕 高效液相色谱 游离棉酚 |
| 英文关键词:cottonseed meal HPLC free gossypol |
| 基金项目: |
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| 摘要点击次数: 1272 |
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| 中文摘要: |
| 建立了棉籽粕中游离棉酚（FG）的高效液相色谱法（HPLC）测定。结果表明，HPLC测定FG的最佳色谱条件为：流动相V（甲醇+0.2%磷酸）∶V（V（乙腈）∶V（水）=1∶3）=85∶15（分别使用2个进样器），柱压16.2 MPa，柱温25℃，流速1.0 mL/min，进样量20 μL，Waters XTerra MS C18不锈钢色谱柱（250 mm×4.6 mm，5 μm），UV检测波长235.5 nm。在最佳条件下，能更好地检测棉籽粕中的游离棉酚，棉酚标准溶液的质量浓度与其峰面积呈正相关，相关系数为0.999 9，在0.15~5 μg/mL之间呈良好线性关系，平均回收率在95.67%～98.99%之间，相对标准偏差在1.28%～2.03%之间。 |
| 英文摘要: |
| The determination method of free gossypol （FG） in cottonseed meal was established by high performance liquid chromatography （HPLC）. The results showed that the optimal HPLC conditions were obtained as follows：mobile phase V（methanol +0.2% H3PO4）∶V（V（acetonitrile）∶V（water ）=1∶3）=85∶15 （respective using two injectors），column pressure 16.2 MPa，column temperature 25℃，flow rate 1.0 mL/min，injection volume 20 μL，Waters XTerra MS C18 stainless steel chromatographic column (250 mm×4.6 mm，5 μm)，UV detection wavelength 235.5 nm. The FG in cottonseed meal could be well detected under the optimal conditions. A good linear relationship between peak area and mass concentration of standard gossypol solution was observed in the range of 0.15-5 μg/mL（R2=0.999 9）. The mean recovery was 95.67%-98.99% with relative standard deviation ranging from 1.28% to 2.03%. |
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