| 袁东强,何 钊,孙 龙,等.丝光绿蝇幼虫油脂提取方法的响应曲面优化[J].中国油脂,2015,40(3):.[YUAN Dongqiang,HE Zhao,SUN Long，etc.Optimization of extraction method of Lucilia sericata larva oil by response surface methodology[J].China Oils and Fats,2015,40(3):.] |
| 丝光绿蝇幼虫油脂提取方法的响应曲面优化 |
| Optimization of extraction method of Lucilia sericata larva oil by response surface methodology |
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| DOI： |
| 中文关键词: 丝光绿蝇 超声波协同微波提取法 浸提法 响应曲面 理化性质 |
| 英文关键词:Lucilia sericata ultrasound-microwave extraction method solvent extraction method response surface methodology physicochemical property |
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| 中文摘要: |
| 为确定丝光绿蝇幼虫油脂的提取方法和性质，为其利用奠定基础，以石油醚为溶剂，采用浸提法和超声波协同微波提取法提取丝光绿蝇幼虫油脂，用响应曲面对2种方法进行优化，得出最佳提取条件，并对油脂的理化性质进行了分析。得到浸提法的最佳提取条件为：温度40℃，时间7.93 h，料液比1∶19.71，理论得率28.27%，实际得率为27.89%；得到超声波协同微波提取法的最佳提取条件为：时间10 min，料液比1∶12，超声波功率339.10 W，微波功率446.64 W，温度57.77℃，理论得率24.69%，实际得率为24.67%。2种方法提取油脂的碘值相近，超声波协同微波提取法油脂的酸值（KOH）较低，为6.55 mg/g；浸提法油脂的过氧化值为6.22 meq/kg；2种油脂颜色饱和值S分别为0.72、0.95，超声波协同微波提取法油脂颜色较深。浸提法提取油脂得率较高，超声波协同微波提取法耗时最短，品质较好。综合分析，超声波协同微波提取法是丝光绿蝇幼虫油脂的适宜提取方法。 |
| 英文摘要: |
| In order to determine the extraction method and property of Lucilia sericata larva oil and lay a foundation for its utilization，the Lucilia sericata larva oil was extracted by solvent extraction method and ultrasound-microwave extraction method respectively with petroleum ether as solvent，then the extraction conditions of the two methods were optimized by response surface methodology，and the physicochemical property of Lucilia sericata larva oil was analyzed. The results showed that the optimal conditions of solvent extraction method were obtained as follows：temperature 40℃，time 7.93 h，ratio of material to solvent 1∶19.71，under these conditions，the theoretical yield and the actual yield were 28.27% and 27.89% respectively; the optimal conditions of ultrasound-microwave extraction method were obtained as follows：time 10 min，ratio of material to solvent 1∶12，ultrasonic power 339.10 W，microwave power 446.64 W，temperature 57.77℃，under these conditions，the theoretical yield and the actual yield were 24.69% and 24.67% respectively. The iodine values of Lucilia sericata larva oils extracted by the two methods were similar，and the acid value of Lucilia sericata larva oil extracted by ultrasound-microwave extraction method was lower，about 6.55 mgKOH/g; the peroxide value of Lucilia sericata larva oil extracted by solvent extraction method was 6.22 meq/kg，the hue-saturation-intensity values S of the two kinds of oils were 0.72 and 0.95 respectively，and the color of Lucilia sericata larva oil extracted by ultrasound-microwave extraction method was deeper. The yield of oil extracted by solvent extraction method was higher，while ultrasound-microwave extraction method consumed less time and the quality of the extracted oil was better.Therefore, ultrasound-microwave extraction method was the appropriate extraction method for Lucilia sericata larva oil. |
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