| 刘 宁,田玉霞,张文杰.无溶剂体系磷脂酶Lecitase® Ultra催化棕榈油甘油解制备甘油二酯研究[J].中国油脂,2015,40(3):.[LIU Ning,TIAN Yuxia,ZHANG Wenjie.Synthesis of diacylglycerol through glycerolysis of palm oil catalyzed by phospholipase Lecitase® Ultra in a solvent-free system[J].China Oils and Fats,2015,40(3):.] |
| 无溶剂体系磷脂酶Lecitase® Ultra催化棕榈油甘油解制备甘油二酯研究 |
| Synthesis of diacylglycerol through glycerolysis of palm oil catalyzed by phospholipase Lecitase® Ultra in a solvent-free system |
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| DOI： |
| 中文关键词: 甘油二酯 棕榈油 磷脂酶Lecitase® Ultra 甘油解 分子蒸馏 |
| 英文关键词:diacylglycerol palm oil phospholipase Lecitase® Ultra glycerolysis molecular distillation |
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| 中文摘要: |
| 采用磷脂酶Lecitase? Ultra在无溶剂体系中催化棕榈油甘油解反应制备甘油二酯（DAG）。采用单因素试验研究了DAG的酶促甘油解反应条件，通过分子蒸馏对DAG进行纯化，并采用GC-MS测定了其脂肪酸组成。结果表明，最佳甘油解工艺参数为：甘油与棕榈油摩尔比7.5∶1，反应温度40℃，初始水分含量4%（占底物总质量），加酶量2%（占底物总质量），反应时间8 h；在最佳条件下，产品中DAG含量为59.2%。经两级分子蒸馏，DAG含量可达88.1%。其中，1，3-DAG含量为1，2-DAG的1.6倍。同原料棕榈油相比，DAG中的饱和脂肪酸得到了显著富集。 |
| 英文摘要: |
| The diacylglycerol （DAG） was prepared through glycerolysis of palm oil catalyzed by phospholipase Lecitase? Ultra in a solvent-free system. The enzyme catalyzed glycerolysis conditions of DAG were studied by single factor experiment and it was purified by molecular distillation，then its fatty acid composition was analyzed by GC-MS. The results showed that the optimal glycerolysis conditions were obtained as follows：molar ratio of glycerol to palm oil 7.5∶1，reaction temperature 40℃，initial water content 4%（based on the total mass of substrates），enzyme dosage 2% （based on the total mass of substrates），and reaction time 8 h. Under the optimal conditions，the content of DAG in the product was 59.2%. While the content of DAG could achieve 88.1% through two-step molecular distillation，in which the content of 1，3-DAG was 1.6 times as high as 1，2-DAG. Compared with palm oil，the saturated fatty acids of DAG were enriched largly. |
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