| 黄行健,章肇敏,杨 芳，等.Saio法提取大豆7S球蛋白的纯化及鉴定[J].中国油脂,2015,40(4):.[HUANG Xingjian,ZHANG Zhaomin,YANG Fang，etc.Purification and identification of soybean 7S globulin extracted by Saio method[J].China Oils and Fats,2015,40(4):.] |
| Saio法提取大豆7S球蛋白的纯化及鉴定 |
| Purification and identification of soybean 7S globulin extracted by Saio method |
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| DOI： |
| 中文关键词: 大豆 7S球蛋白 纯化 鉴定 |
| 英文关键词:soybean 7S globulin purification identification |
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| 中文摘要: |
| 研究利用Sephadex G-200凝胶柱层析法和纤维素DE-52离子交换柱层析法分离纯化经过Saio法提取的大豆7S球蛋白样品，采用SDS-聚丙烯酰胺凝胶电泳和高效毛细管电泳进一步鉴定Saio法提取的大豆7S球蛋白样品的组分及含量。结果表明，Saio法提取的大豆7S球蛋白纯度约为81%。 |
| 英文摘要: |
| The soybean 7S globulin extracted by Saio method was separated and purified by the methods of Sephadex G-200 gel chromatography and cellulose DE-52 ion-exchange chromatography, and its component and content were further identified by SDS-PAGE and high performance capillary electrophoresis. The results showed that the purity of soybean 7S globulin extracted by Saio method was about 81%. |
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