| 何晓梅,陈存武,乔德亮，等.芝麻素的超声辅助提取技术及生物活性研究[J].中国油脂,2015,40(5):.[HE Xiaomei,CHEN Cunwu,QIAO Deliang，etc.Ultrasound-assisted extraction and bioactivity of sesamin[J].China Oils and Fats,2015,40(5):.] |
| 芝麻素的超声辅助提取技术及生物活性研究 |
| Ultrasound-assisted extraction and bioactivity of sesamin |
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| DOI： |
| 中文关键词: 芝麻 芝麻素 超声辅助提取 还原能力 抗菌活性 |
| 英文关键词:sesame sesamin ultrasound-assisted extraction reducing activity antibacterial activity |
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| 摘要点击次数: 1223 |
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| 中文摘要: |
| 运用超声辅助90%乙醇溶液提取芝麻素。在单因素实验的基础上，运用正交实验优化芝麻素超声辅助提取工艺参数。普鲁士蓝法测定芝麻素还原能力，滤纸片-抑菌圈法测定其抗菌活性。结果表明：超声辅助提取的最优条件为超声功率300 W、液固比25∶1、超声温度55℃、超声时间25 min，在最优条件下，芝麻素提取率为(3.942±0.085)mg/g；芝麻素具有一定的还原能力，但比相同质量浓度的VC弱得多；当质量浓度大于40 mg/mL时，芝麻素对蜡状芽孢杆菌、金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌均有不同程度的抑制作用，质量浓度大于60 mg/mL时其对4种细菌抑制作用的强弱顺序为蜡状芽孢杆菌＞金黄色葡萄球菌＞枯草芽孢杆菌＞大肠杆菌，而当质量浓度小于100 mg/mL时，芝麻素对真菌黄曲霉无抑制作用。 |
| 英文摘要: |
| Sesamin was extracted by ultrasound-assisted 90% ethanol solution. On the basis of single factor experiment, the parameters of the ultrasound-assisted extraction of sesamin were optimized by orthogonal experiment. The reducing activity and the antibacterial activity of sesamin were determined by Prussian blue method and filter paper-inhibition zone method, respectively. The results showed that the optimal ultrasound-assisted extraction conditions were obtained as follows:ultrasonic power 300 W, ratio of liquid to solid 25∶1, ultrasonic temperature 55℃ and ultrasonic time 25 min. Under the optimal conditions, the extraction rate of sesamin was (3.942±0.085) mg/g. Sesamin had certain reducing activity, much weaker than VC of the same mass concentration. When the mass concentration of sesamin was higher than 40 mg/mL, sesamin showed different degrees of inhibitory action on Bacillus cereus,Staphylococcus aureus,Bacillus subtilis and Escherichia coli, and the inhibitory action of sesamin with mass concentration higher than 60 mg/mL on Bacillus cereus was the strongest, followed by Staphylococcus aureus,Bacillus subtilis and Escherichia coli. When the mass concentration of sesamin was less than 100 mg/mL, no obvious inhibitory action of sesamin on Aspergillus flavus was observed. |
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