| 徐启峻,滕宏飞,黄 程,等.一株柱状假丝酵母产脂肪酶条件的优化研究[J].中国油脂,2015,40(5):.[XU Qijun,TENG Hongfei,HUANG Cheng，etc.Optimization of production conditions of lipase by Candida cylindracea[J].China Oils and Fats,2015,40(5):.] |
| 一株柱状假丝酵母产脂肪酶条件的优化研究 |
| Optimization of production conditions of lipase by Candida cylindracea |
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| DOI： |
| 中文关键词: 柱状假丝酵母 酸性脂肪酶 生长 产酶 |
| 英文关键词:Candida cylindracea acid lipase growth enzyme production |
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| 摘要点击次数: 1084 |
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| 中文摘要: |
| 对一株柱状假丝酵母菌的产酶条件进行了优化。确定最佳培养基配方为橄榄油6 g/L、酵母膏9 g/L、吐温-80 1%、CaCl20.5 g/L、K2HPO4 2 g/L、MgSO4·7H2O2 g/L、pH自然；确定最优发酵条件为培养基初始pH 5.2、培养时间25 h、摇床转速150 r/min。在最适产酶条件下，重复发酵5批后柱状假丝酵母所产脂肪酶平均酶活在（15.00±0.05）U/mL，RSD为2.34%，重复性较好。该株酵母菌生存环境为酸性，并在pH为5.2时产酶效果最好，推导该脂肪酶为酸性脂肪酶，对油脂水解产生的游离脂肪酸将会有一定程度的抵抗作用。此外，柱状假丝酵母培养25 h后即可获得最佳产酶效果，发酵周期短，既能节约成本，又能提高单位时间产酶量，适用于工业化生产。 |
| 英文摘要: |
| The production conditions of lipase by Candida cylindracea were optimized. The best formular of the medium were obtained as follows: olive oil 6 g/L, yeast extract 9 g/L, tween-80 1%, CaCl20.5 g/L,K2HPO4 2 g/L,MgSO4·7H2O2 g/L, natural pH. The optimal fermentation conditions were obtained as follows: initial pH of medium 5.2, cultural time 25 h, speed of shaker 150 r/min. Under the optimal conditions, the average activity of the lipase produced by Candida cylindraceawas （15.00±0.05）U/mL after fermentation for 5 batches and the relative standard deviation was 2.34%, which had an ideal repeatability. The Candida cylindracea strain lived in acid environment and the production efficient of lipase was the best at pH 5.2, therefore, it was inferred that the lipase was acid lipase, which would have a certain degree of resistance on free fatty acids from the hydrolysis of oils and fats. Moreover, the highest activity of lipase produced by Candida cylindracea could be achieved after it was fermented for 25 h, which had the items of short fermentation period, low cost and high unit time productivity of lipase, suitable for industrial production. |
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