| 周建中,高 蕾,陶永霞.棉籽7S及12S球蛋白的分离纯化及其物化性质的研究[J].中国油脂,2015,40(6):.[ZHOU Jianzhong,GAO Lei,TAO Yongxia.Isolation and purification of cottonseed 7S and 12S globulins and their physicochemical properties[J].China Oils and Fats,2015,40(6):.] |
| 棉籽7S及12S球蛋白的分离纯化及其物化性质的研究 |
| Isolation and purification of cottonseed 7S and 12S globulins and their physicochemical properties |
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| DOI： |
| 中文关键词: 棉籽球蛋白 7S 12S 分离纯化 物化性质 |
| 英文关键词:cottonseed globulin 7S 12S isolation and purification physicochemical property |
| 基金项目: |
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| 中文摘要: |
| 以棉籽贮存蛋白为原料，采用蛋白纯化系统（配备凝胶层析柱）对棉籽7S和12S球蛋白进行分离纯化，对所得7S和12S球蛋白的蛋白质含量、氨基酸组成、变性温度及荧光强度等物化性质进行了测定分析。结果表明：棉籽7S及12S球蛋白的蛋白质含量分别为（91.59±0.53）%和（93.35±0.62）%，其SDS-PAGE谱图中除显示各自特征条带外无其他杂带，其氨基酸组成与绝大部分油料种子中球蛋白的基本一致，含有丰富的谷氨酸、天冬氨酸和精氨酸，而半胱氨酸和甲硫氨酸含量较低；差示扫描量热仪（DSC）分析棉籽7S及12S球蛋白的变性温度分别为93.3℃和107.7℃；棉籽7S及12S球蛋白的荧光发射光谱最大荧光强度均在325 nm左右。 |
| 英文摘要: |
| Cottonseed 7S and 12S globulins were isolated and purified from cottonseed storage protein by protein purification system coupled with gel chromatography,and the protein contents, amino acid compositions, denaturation temperatures and fluorescence intensities of 7S and 12S globulins obtained were determined and analyzed. The results showed that the protein contents of cottonseed 7S and 12S globulins were （91.59±0.53）% and （93.35±0.62）% respectively, and there was no impurity band existed in the SDS-PAGE chromatography of cottonseed 7S and 12S globulins except for the characteristic bands; cottonseed 7S and 12S globulins were rich in Glu, Asp and Arg, and poor in Cys and Met, which was similar to that of most oil seeds; the differential scanning calorimetry（DSC）results indicated that the denaturation temperatures of cottonseed 7S and 12S globulins were 93.3℃ and 107.7℃, respectively; and the maximum fluorescence intensities of cottonseed 7S and 12S globulins were both at about 325 nm. |
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