| 张淑青,潘丽军,姜绍通，等.非水相酶促合成L-抗坏血酸辛酸酯及其结构表征[J].中国油脂,2015,40(8):.[ZHANG Shuqing,PAN Lijun,JIANG Shaotong，etc.Lipase-catalyzed synthesis of L-ascorbyl octanoate in non-aqueous phase and its structural characterization[J].China Oils and Fats,2015,40(8):.] |
| 非水相酶促合成L-抗坏血酸辛酸酯及其结构表征 |
| Lipase-catalyzed synthesis of L-ascorbyl octanoate in non-aqueous phase and its structural characterization |
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| DOI： |
| 中文关键词: 正辛酸 L-抗坏血酸 脂肪酶 L-抗坏血酸辛酸酯 结构表征 |
| 英文关键词:n-octanoic acid L-ascorbic acid lipase L-ascorbyl octanoate structural characterization |
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| 摘要点击次数: 1003 |
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| 中文摘要: |
| 以中链脂肪酸正辛酸为酰基供体，在有机相体系中利用脂肪酶催化酯化反应合成L-抗坏血酸辛酸酯。根据初步试验结果，选取对L-抗坏血酸酯化转化率影响显著的因素，进行响应面试验设计，建立回归模型，得到最优参数组合。结果表明：反应时间、反应温度、反应时间和反应温度的交互作用以及反应时间和底物浓度的交互作用对L-抗坏血酸酯化转化率均有显著影响(p＜0.05)，回归模型可用。L-抗坏血酸辛酸酯酶法合成最优工艺条件为反应时间11.45 h、底物(L-抗坏血酸)浓度0.24 mol/L、反应温度55.3℃、脂肪酶用量(以L-抗坏血酸质量计)19.18%，此条件下L-抗坏血酸酯化转化率可达83.31%。产物经IR、MS、1H NMR和13C NMR结构表征，证实正辛酸被引入到了L |
| 英文摘要: |
| L-ascorbyl octanoate was synthesized through lipase-catalyzed esterification in organic system with mid-chain fatty acid n-octanoic acid as acyl donor. The reaction parameters that highly influenced the conversion rate of L-ascorbic acid were selected as main variables to design the response surface experiment on the basis of preliminary experiments, then a regression model was established and the optimal synthesis conditions were obtained. The results indicated that reaction time, reaction temperature, interaction between reaction time and reaction temperature and interaction between reaction time and substrate concentration had significant effects on the conversion rate of L-ascorbic acid (p＜0.05), and the regression model could be used to predict the conversion rate of L-ascorbic acid. The optimal enzymatic synthesis conditions of L-ascorbyl octanoate were obtained as follows: reaction time 11.45 h, substrate(L-ascorbic acid) concentration 0.24 mol/L, reaction temperature 55.3℃, dosage of lipase (based on the mass of L-ascorbic acid) 19.18%. Under these conditions,the conversion rate of L-ascorbic acid reached 83.31%. In addition, the product was characterized by IR, MS, 1H NMR and 13C NMR,and the result showed that n-octanoic acid was introduced into the sixth hydroxyl of L-ascorbic acid. |
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