| 代志凯,李祥清,赵 健，等.硅胶柱层析法纯化生育酚和生育三烯酚的工艺研究[J].中国油脂,2016,41(9):.[DAI Zhikai,LI Xiangqing,ZHAO Jian，etc.Purification of tocopherols and tocotrienols by silica gel column chromatography[J].China Oils and Fats,2016,41(9):.] |
| 硅胶柱层析法纯化生育酚和生育三烯酚的工艺研究 |
| Purification of tocopherols and tocotrienols by silica gel column chromatography |
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| DOI： |
| 中文关键词: 生育酚 生育三烯酚 棕榈油脱臭馏出物 硅胶柱层析 |
| 英文关键词:tocopherols tocotrienols palm oil deodorizer distillate silica gel column chromatography |
| 基金项目: |
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| 摘要点击次数: 1390 |
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| 中文摘要: |
| 通过硅胶柱层析法分离纯化棕榈油脱臭馏出物来源的天然VE，试验中以天然VE含量和回收率为评价指标，考察洗脱剂、洗脱体积、洗脱流速及载样量对硅胶柱层析分离的影响。结果表明：分别利用1 BV的环己烷、1 BV5%甲基叔丁基醚-环己烷、1 BV 30%甲基叔丁基醚-环己烷及2 BV的甲基叔丁基醚梯度洗脱，在洗脱流速0.75 BV/h、载样量为硅胶质量15%的条件下，硅胶柱层析提纯后的天然VE含量可由原来的8.17%提高到52.29%，回收率达80%以上，为棕榈油脱臭馏出物中的天然VE分离纯化提供了一种简便、高效的分离方法。 |
| 英文摘要: |
| Natural vitamin E was separated and purified from palm oil deodorizer distillate by silica gel column chromatography. The effects of eluent，elution volume，flow rate of elution and loading amount on separation efficiency were investigated with content and recovery rate of natural vitamin E as evaluation indexes. The results showed that the optimal separation conditions were obtained as follows：with 1 BV cyclohexane，1 BV 5% methyl tert-butyl ether-cyclohexane，1 BV 30% methyl tert-butyl ethe-cyclohexane and 2 BV methyl tert-butyl ether as gradient eluent，flow rate of elution 0.75 BV/h and loading amount 15% of silica gel mass. Under the optimal conditions，the content of natural vitamin E after purification by silica gel column chromatography increased from 8.17% to 52.29% with recovery rate above 80%. It provided a simple and efficiency method for the separation and purification of natural vitamin E from palm oil deodorizer distillate. |
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