| 罗国平，梁宇柱，闫梦茹，等.HPLC法测定牡丹籽油中游离型和结合型α-亚麻酸、亚油酸及油酸的含量[J].中国油脂,2017,42(8):.[LUO Guoping，LIANG Yuzhu，YAN Mengru，et al.Determination of contents of free and conjugated α-linolenic acid, linoleicacid and oleic acid in Paeonia suffruticosa Andr. seed oil by HPLC[J].China Oils and Fats,2017,42(8):.] |
| HPLC法测定牡丹籽油中游离型和结合型α-亚麻酸、亚油酸及油酸的含量 |
| Determination of contents of free and conjugated α-linolenic acid, linoleicacid and oleic acid in Paeonia suffruticosa Andr. seed oil by HPLC |
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| DOI： |
| 中文关键词: 牡丹籽油 α-亚麻酸 亚油酸 油酸 高效液相色谱法 |
| 英文关键词:Paeonia suffruticosa Andr. seed oil 伪-linolenic acid linoleic acid oleic acid HPLC |
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| 摘要点击次数: 1360 |
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| 中文摘要: |
| 采用高效液相色谱（HPLC）法测定牡丹籽油中游离型、结合型α-亚麻酸、亚油酸及油酸的含量。检测条件为Agilent C18高效色谱柱(3.0 mm×50 mm， 2.7 μm)，流动相乙腈-0.1%磷酸水溶液（体积比85∶15），流速1 mL/min，检测波长203 nm，柱温35?℃，进样量20 μL。结果表明，该方法精密度、重复性、稳定性良好。α-亚麻酸在41.4～207 μg/mL范围内，其峰面积与质量浓度呈良好的线性关系；亚油酸在20.2～101 μg/mL范围内，其峰面积与质量浓度呈良好的线性关系；油酸在14.9～74.6 μg/mL范围内，其峰面积与质量浓度呈良好的线性关系。牡丹籽油样品中，游离型α-亚麻酸、亚油酸和油酸的平均含量分别为4.47、1.89 mg/mL和0.92 mg/mL，结合型α-亚麻酸、亚油酸和油酸的平均含量分别为437、237 mg/mL和156 mg/mL。建立的方法具有分离效率高、选择性好和条件温和的优点。 |
| 英文摘要: |
| The contents of free and conjugated α-linolenic acid, linoleic acid and oleic acid in Paeonia suffruticosa Andr. seed oil were determined by HPLC. The detection conditions were as follows: Agilent C18 column (3.0 mm×50 mm, 2.7 μm), mobile phase acetonitrile-0.1% phosphoric acid solution (volume ratio 85∶15), flow rate 1 mL/min, detection wavelength 203 nm, column temperature 35℃ and injection volume 20 μL. The results showed that the method had good precision, repeatability and stability. Good linear relationships of α-linolenic acid, linoleic acid and oleic acid were achieved in the mass concentration ranges of 41.4-207 μg/mL, 20.2-101 μg/mL and 14.9-74.6 μg/mL, respectively. The average contents of free α-linolenic acid, linoleic acid and oleic acid were 4.47，1.89 mg/mL and 0.92 mg/mL respectively, and the average contents of conjugated α-linolenic acid, linoleic acid and oleic acid were 437，237 mg/mL and 156 mg/mL respectively in Paeonia suffruticosa Andr.seed oil. The methods had the advantages of high separation efficiency, good selectivity and mild conditions. |
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