| 孙林，刘平，卓伟伟，方 等.微生物发酵生产高蛋白多肽菜籽粕的研究[J].中国油脂,2017,42(12):.[SUN Lin，LIU Ping,ZHUO Weiwei,et al.Production of high protein and polypeptide rapeseed meal by microbial fermentation[J].China Oils and Fats,2017,42(12):.] |
| 微生物发酵生产高蛋白多肽菜籽粕的研究 |
| Production of high protein and polypeptide rapeseed meal by microbial fermentation |
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| DOI： |
| 中文关键词: 菜籽粕 粗蛋白质 多肽 发酵 |
| 英文关键词:rapeseed meal crude protein polypeptide fermentation |
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| 中文摘要: |
| 以菜籽粕为原料，以菜籽粕粗蛋白质含量和多肽含量为评价指标，通过植物乳酸菌、黑曲霉菌、枯草芽孢杆菌的单菌发酵和混菌发酵实验，进行最佳菌种组合和发酵条件的研究。结果表明：单菌发酵黑曲霉菌效果最好，菜籽粕粗蛋白质含量和多肽含量分别为38.4%和3.682%；混菌发酵最佳菌种组合为植物乳酸菌、黑曲霉菌、枯草芽孢杆菌，其接种量分别为4%、6%、6%，优化发酵条件为水料比1.7∶?1、34?℃下发酵48 h。在上述条件下菜籽粕粗蛋白质含量和多肽含量分别为42.5% 和7.86%, 硫甙含量降至21.637 μmol/g、植酸含量为0.27%、单宁含量为0.41 mg/g，得到高蛋白、多肽、低毒的菜籽粕饲料。 |
| 英文摘要: |
| With rapeseed meal as raw material, the contents of crude protein and polypeptide as evaluation indexes, the strain combination and fermentation conditions were studied by single strain fermentation and multi-strains(Lactobacillus plantarum, Aspergillus niger, Bacillus subtilis) fermentation. The results showed that the contents of crude protein and polypeptide in rapeseed meal fermented by Aspergillus niger were the highest by single strain fermentation, reaching 38.4% and 3.682% respectively.The optimal inoculation amounts of Lactobacillus plantarum, Aspergillus niger, Bacillus subtilis were 4%, 6% and 6% respectively, and the optimal fermentation conditions were obtained as follows: ratio of water to material 1.7∶?1, fermentation temperature 34?℃ and fermentation time 48 h. Under these conditions, the contents of crude protein and polypeptide were 42.5% and 7.86% respectively. After fermentation, glucosinolate content reduced to 21.637 μmol/g, and the contents of phytic acid and tannin were 0.27% and 0.41 mg/g respectively. High protein, high polypeptide and low toxicity rapeseed meal feed was obtained. |
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