| 闫荣玲，毛龙毅，廖阳，等.薄荷种子油超声辅助提取工艺及其脂肪酸组成、理化性质与急性毒性分析[J].中国油脂,2018,43(5):.[YAN Rongling，MAO Longyi，LIAO Yang，etc.Ultrasound-assisted extraction of seed oil from Mentha haplocalyx and its fatty acid composition, physicochemical properties and acute toxicity analysis[J].China Oils and Fats,2018,43(5):.] |
| 薄荷种子油超声辅助提取工艺及其脂肪酸组成、理化性质与急性毒性分析 |
| Ultrasound-assisted extraction of seed oil from Mentha haplocalyx and its fatty acid composition, physicochemical properties and acute toxicity analysis |
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| DOI： |
| 中文关键词: 薄荷 种子油 超声辅助 提取工艺 理化性质 脂肪酸组成 急性毒性 |
| 英文关键词:Mentha haplocalyx seed oil ultrasound-assist extraction process physicochemical property fatty acid composition acute toxicity |
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| 摘要点击次数: 1420 |
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| 中文摘要: |
| 以正己烷为提取剂，采用单因素实验及正交实验优化得到超声辅助提取薄荷种子油的最佳工艺，并对所得薄荷种子油进行脂肪酸组成、理化性质及急性毒性分析。结果表明：薄荷种子油的最佳提取工艺为料液比1∶?10、超声时间50 min、超声功率300 W、超声温度50?℃，此条件下薄荷种子油得率为25.6%；薄荷种子油经GC-MS分析共检测到4种脂肪酸，分别是软脂酸(5.44%)、油酸(13.83%)、亚油酸(23.85%)、亚麻酸(56.88%)，不饱和脂肪酸含量达到94.56%，约为饱和脂肪酸含量的17.4倍；薄荷种子油的密度、折光指数（n20D）、碘值（I）、酸值（KOH）、皂化值（KOH）分别为0.908 g/mL、1.511、148.6 g/100 g、0.87 mg/g、182.7 mg/g；薄荷种子油对DPPH自由基表现出显著清除效应，清除率随体积分数增加而增加但增幅逐渐减小；急性毒性实验发现，小鼠在实验期内未出现行为、体征、脏器异常及死亡现象。薄荷种子油是一种不饱和脂肪酸含量高、不表现急性毒性、开发潜质大的优质植物油脂资源。 |
| 英文摘要: |
| The optimal ultrasound-assisted extraction process of seed oil from Mentha haplocalyx was obtained through single factor experiment and orthogonal experiment with n-hexane as extraction solvent, then the fatty acid composition, physicochemical properties and acute toxicity of Mentha haplocalyx seed oil were analyzed. The results showed that the optimal extraction process of seed oil from Mentha haplocalyx was obtained as follows: ratio of material to liquid 1∶?10, ultrasonic time 50 min, ultrasonic power 300 W and ultrasonic temperature 50?℃. Under these conditions, the yield of seed oil could reach 25.6%. GC-MS analysis revealed that there were four kinds of fatty acids in Mentha haplocalyx seed oil, which were palmitic acid (544%), oleic acid (13.83%), linoleic acid (23.85%) and linolenic acid (56.88%). The unsaturated fatty acids accounted for 94.56%, which was about 17.4 times of saturated fatty acid content. The density, refractive index(n20D), iodine value, acid value and saponification value of the seed oil were 0.908 g/mL, 1.511, 148.6 gI/100 g, 087 mgKOH/g and 182.7 mgKOH/g, respectively. Mentha haplocalyx seed oil showed a significant scavenging effect on DPPH radical, and the scavenging rate increased with the rising of oil volume fraction while the increase degree decreased gradually. Acute toxicological test found that the mice did not show any behavior, signs, organ abnormalities and death during the experimental period. Mentha haplocalyx seed oil was a good vegetable oil resource with high content of unsaturated fatty acids and great development potential, and it did not exhibit acute toxicity. |
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