| 董训赞，车绕琼，赵永腾，等.黄腐酸对单针藻Monoraphidium sp.FXY-10油脂积累及me、pepc、accD基因表达量的影响[J].中国油脂,2018,43(7):.[DONG Xunzan, CHE Raoqiong, ZHAO Yongteng, etc.Effects of fulvic acid on lipid accumulation and genes (me, pepc, accD ) expression of Monoraphidium sp. FXY-10[J].China Oils and Fats,2018,43(7):.] |
| 黄腐酸对单针藻Monoraphidium sp.FXY-10油脂积累及me、pepc、accD基因表达量的影响 |
| Effects of fulvic acid on lipid accumulation and genes (me, pepc, accD ) expression of Monoraphidium sp. FXY-10 |
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| DOI： |
| 中文关键词: Monoraphidium sp. FXY-10 黄腐酸 油脂 ROS 基因表达 |
| 英文关键词:Monoraphidium sp. FXY-10 fulvic acid lipid ROS gene expression |
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| 中文摘要: |
| 研究了黄腐酸对单针藻Monoraphidium sp. FXY-10生长、油脂含量、活性氧（ROS）水平及与油脂合成相关基因表达的影响。采用两阶段培养方法（第一阶段异养培养，第二阶段自养培养），并添加黄腐酸诱导。结果表明：在25 mg/L黄腐酸诱导下，6 d时藻细胞中油脂含量可达54.3%，是对照组的1.12倍,藻细胞内活性氧水平是对照组的1.46倍。此外，油脂合成相关酶基因与油脂合成存在相关性，黄腐酸诱导下，me、accD、pepc基因表达量分别是对照组的1.32倍（4 d）、1.67倍（4 d）及1.71倍（6 d）。研究表明，黄腐酸提高油脂积累可能与活性氧水平及基因表达有关。 |
| 英文摘要: |
| The effects of fulvic acid on the biomass, lipid content, reactive oxygen(ROS) level and the expression of lipid biosynthesis-related genes of Monoraphidium sp. FXY-10 were studied. Two stage culture method(first heterotrophic and then photoautotrophic) was used and fulvic acid was added to induce. The results showed that the lipid content reached 54.3% in algal cells treated with 25 mg/L fulvic acid, which was 1.12 times of the control, and the intracellular ROS level was 1.46 times of the control. Moreover, the lipid biosynthesis-related genes was correlated with the lipid synthesis, and the expressions of me, accD and pepc were 1.32 times(4 d), 1.67 times(4 d) and 1.71 times(6 d) of the control. These results demonstrated that fulvic acid significantly increased microalgae lipid accumulation by changing the ROS level and gene expression. |
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