| 王丽娟，杨宋琪，丁丽梅，等.3种不同培养基对斜生栅藻生长和油脂积累的影响[J].中国油脂,2018,43(9):.[WANG Lijuan, YANG Songqi, DING Limei, etc.Effects of three different culture mediums on growth and lipid accumulation of Scenedesmus obliquus[J].China Oils and Fats,2018,43(9):.] |
| 3种不同培养基对斜生栅藻生长和油脂积累的影响 |
| Effects of three different culture mediums on growth and lipid accumulation of Scenedesmus obliquus |
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| DOI： |
| 中文关键词: 斜生栅藻 培养基 生长水平 油脂 脂肪酸 |
| 英文关键词:Scenedesmus obliquus culture medium growth level lipid fatty acid |
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| 摘要点击次数: 1480 |
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| 中文摘要: |
| 以斜生栅藻FACHB-12为原料，采用BG11、F/2和SE 3种培养基对其培养。通过对生物量、生长速率、油脂含量、油脂组分以及脂肪酸组成的测定，比较不同培养基对斜生栅藻生长和油脂积累的影响。结果表明：BG11培养基更有利于斜生栅藻的生长，而F/2培养基更适合油脂以及中性脂的积累；斜生栅藻在BG11培养基中比生长速率为0.095，培养15 d生物量为0.36 g/L，叶绿素a含量达到1.23 mg/L；而在F/2培养基中油脂含量可达细胞干重的24.75%，其中中性脂占总脂的36.64%，产率达0.98 mg/（L·d）；并且在F/2培养基中斜生栅藻脂肪酸组成以C16∶?0和C18∶?1为主，含量分别占总脂肪酸的25.84%和54.47%，更适合作为制备生物柴油的原料。 |
| 英文摘要: |
| Three kinds of culture mediums (BG11, F/2 and SE) were used to culture Scenedesmus obliquus FACHB-12. The effects of different culture mediums on growth and lipid accumulation of Scenedesmus obliquus were investigated by determining its biomass, growth rate, lipid content, lipid fraction distribution and fatty acid composition. The results showed that BG11 medium was more conducive to the growth of Scenedesmus obliquus, while the F/2 medium was more suitable for the lipid and neutral lipid accumulation. The specific growth rate, biomass and chlorophyll a content of Scenedesmus obliquus were respectively 0.095, 0.36 g/L and 1.23 mg/L after being cultured for 15 d in BG11 medium. The lipid content of Scenedesmus obliquus reached 24.75% of dry cell weight in Scenedesmus obliquusin F/2 medium, in which the neutral lipid accounted for 36.64% and its productivity was 0.98 mg/(L·d). In addition, the C16∶?0 and C18∶?1 was main fatty acids in Scenedesmus obliquusin in F/2 medium, which accounted for 25.84% and 54.47% of the total fatty acids respectively. Therefore, it was an ideal raw material for biodiesel. |
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