| 陈丹阳，韩涛，杜斌，等.酶解蚕豆蛋白制备降胆固醇肽及其响应面优化[J].中国油脂,2018,43(10):.[CHEN Danyang，HAN Tao，DU Bin，etc.Optimization of preparation of hypocholesterolemic peptides from broad bean protein by enzymatic hydrolysis using response surface methodology[J].China Oils and Fats,2018,43(10):.] |
| 酶解蚕豆蛋白制备降胆固醇肽及其响应面优化 |
| Optimization of preparation of hypocholesterolemic peptides from broad bean protein by enzymatic hydrolysis using response surface methodology |
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| DOI： |
| 中文关键词: 蚕豆 蛋白质 酶解 胆固醇 多肽 |
| 英文关键词:broad bean protein enzymatic hydrolysis cholesterol peptides |
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| 中文摘要: |
| 为了得到具有较高降胆固醇活性的蚕豆肽，对蚕豆蛋白的酶解条件进行了研究。通过比较6种蛋白酶对蚕豆蛋白的酶解程度以及降胆固醇能力，选择碱性蛋白酶进一步优化酶解条件。以水解度（DH）与降胆固醇能力为指标，利用单因素试验和响应面试验优化确定了酶解蚕豆蛋白制备降胆固醇肽的工艺参数。结果表明，最佳酶解参数为：酶解温度40?℃，pH 9.0，酶解时间60 min，加酶量8 000 U/g，料液比1∶?20。验证试验后得到的水解度为14.29%蚕豆多肽产品，其对胆酸钠、甘氨胆酸钠、牛磺胆酸钠抑制率分别为52.98%、31.87%、25.17%，与理论模型预测值52.49%、3144%、24.76%基本相符。 |
| 英文摘要: |
| The hypocholesterolemic peptides from broad bean protein by enzymatic hydrolysis were investigated. In order to obtain broad bean peptides with high cholesterol-lowering activity，six proteinases were compared on the degree of hydrolysis (DH) of broad bean protein and the hypocholesterolemic abilitity, and alkaline protease was employed. Then the DH and hypocholesterolemic ability were used as indicators, single factor experiment and response surface methodology were applied to get the optimal enzymatic hydrolysis parameters of broad bean protein.The results showed that the optimal enzymatic hydrolysis parameters for alkaline protease were obtained as follows: enzymolysis temperature 40?℃, pH 9.0, enzymolysis time 60 min, dosage of enzyme 8 000 U/g and ratio of material to liquid 1∶?20. Under the optimal conditions, broad bean peptides with 1429% DH could be obtained, and its inhibition rates on sodium cholate, sodium glycine and sodium taurocholate were predicted 52.49%, 31.44% and 24.76%, and the validation tests gave 52.98%, 31.87% and 25.17%, respectively. |
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