| 王皖婧，何钊.反式棕榈油酸对肥胖小鼠代谢稳态的作用研究[J].中国油脂,2018,43(10):.[WANG Wanjing，HE Zhao.Effects of trans-palmitoleic acid on metabolic homeostasis of obese mice[J].China Oils and Fats,2018,43(10):.] |
| 反式棕榈油酸对肥胖小鼠代谢稳态的作用研究 |
| Effects of trans-palmitoleic acid on metabolic homeostasis of obese mice |
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| DOI： |
| 中文关键词: 反式棕榈油酸 肥胖 葡萄糖敏感性 进食量 能量代谢 |
| 英文关键词:trans-palmitoleic acid obesity glucose sensitivity food intake energy metabolism |
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| 中文摘要: |
| 为了探讨反式棕榈油酸对肥胖小鼠代谢稳态的影响,利用高脂诱导的肥胖模型，观察反式棕榈油酸对小鼠的体重、进食量、空腹血糖、餐后血糖、葡萄糖敏感性、呼吸交换速率（RER）、产热和活动量作用。并采用QPCR和Western BLOT方法分别检测小鼠脂肪代谢相关基因和肝脏炎症相关蛋白质的表达。结果显示，反式棕榈油酸在不影响肥胖小鼠体重、空腹血糖、餐后血糖和肝脏炎症的情况下，可以显著改善葡萄糖敏感性（p<0.01）、抑制食欲（p<0.000 1）、降低皮下脂肪的积累（p<0.05）、增强呼吸代谢（p<0.000 1）和产热（p<0.000 1），但同时动物活动量也显著减少（p<0.001），从而达到一种能量平衡的状态。由此可知，反式棕榈油酸虽然是一种反式脂肪酸，但对肥胖小鼠代谢并无显著有害影响，可为今后筛选安全的膳食食品添加剂研究提供一定的参考。 |
| 英文摘要: |
| In order to investigate the effects of trans-palmitoleic acid on metabolic homeostasis of obese mice, an obesity model induced by high-fat diet was established, and the body weight, food intake, fasting glucose, postprandial glucose, glucose sensitivity, respiratory exchange ratio (RER), heat production and activity of the mice were examined. QPCR and Western BLOT were respectively used to detect the expression of lipid-metabolism-related genes and liver inflammation-related proteins of mice. The results showed that trans-palmitoleic acid significantly improved glucose sensitivity (p<0.01), inhibited appetite (p<0.000 1), reduced subcutaneous fat accumulation (p<0.05),and enhanced respiratory metabolism (p<0.000 1) and heat production (p<0.000 1) without affecting body weight, fasting blood glucose, postprandial glucose and liver inflammation of mice, while it significantly decreased animal activity (p<0.001), finally leading to an energy balance status. In conclusion, it suggested that although as a kind of trans fatty acids, trans-palmitoleic acid had no harmful effect on the metabolism of obese mice, so it could provide a certain reference value for future research on safe dietary food additives. |
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