| 芦鑫，姜梦楠，张丽霞，等.制备芝麻抗氧化肽的蛋白酶筛选[J].中国油脂,2018,43(11):.[LU Xin, JIANG Mengnan, ZHANG Lixia，etc.Screening of protease for preparing antioxidant peptide from sesame protein[J].China Oils and Fats,2018,43(11):.] |
| 制备芝麻抗氧化肽的蛋白酶筛选 |
| Screening of protease for preparing antioxidant peptide from sesame protein |
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| DOI： |
| 中文关键词: 芝麻蛋白 抗氧化肽 蛋白酶 逼近理想解排序法 |
| 英文关键词:sesame protein antioxidant peptide protease technique for order preference by similarity to ideal solution |
| 基金项目: |
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| 摘要点击次数: 1388 |
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| 中文摘要: |
| 研究了菠萝蛋白酶、木瓜蛋白酶、中性蛋白酶、胰蛋白酶、碱性蛋白酶2709和Alcalase蛋白酶分别酶解芝麻蛋白制备芝麻抗氧化肽，测定多肽产率、水解度并评价抗氧化活性，以多肽产率和抗氧化活性为参数采用逼近理想解排序法对酶解物进行排序，确定适宜制备芝麻抗氧化肽的蛋白酶。结果表明：蛋白酶品种差异会显著影响芝麻抗氧化肽的制备效果；在加酶量相同时，碱性蛋白酶2709、Alcalase蛋白酶、胰蛋白酶酶解芝麻蛋白能力强，多肽产率高，且抗氧化活性强；根据与最优向量距离总和，用于制备芝麻抗氧化肽蛋白酶的适宜性由高到低依次为碱性蛋白酶2709、Alcalase蛋白酶、胰蛋白酶、中性蛋白酶、菠萝蛋白酶、木瓜蛋白酶。 |
| 英文摘要: |
| To select the suitable protease hydrolyzing sesame protein to generate antioxidant peptide, the yields of peptide, degrees of hydrolysis and antioxidant capacities were determined during hydrolyzing sesame protein by Bromelain, Papain, Neutrase, Trypsin, alkaline protease 2709 and Alcalase, then the yield of peptide and antioxidant capacities were analyzed by technique for order preference by similarity to ideal solution to ascertain the order of hydrolysate derived from six proteases. The results showed that varieties difference of protease significantly influenced the performance of antioxidant peptide prepared from sesame protein. When the enzyme addition was the same, high degree of hydrolysis and yield of peptide were observed in hydrolyzing sesame protein by alkaline protease 2709, Alcalase and Trypsin, and their hydrolysates showed high antioxidant activity. According to the sum of distance of optimal vector, the suitable protease for preparation of antioxidant peptide from sesame protein from high to low was obtained as follows: alkaline protease 2709, Alcalase, Trypsin, Neutrase, Bromelain and Papain. |
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