| 王铁良,魏亮亮,刘冰杰，等.杜马斯燃烧法测定大豆水溶性蛋白含量的方法研究[J].中国油脂,2019,44(1):.[WANG Tieliang,WEI Liangliang, LIU Bingjie，etc.Method for determination of water-soluble protein content in soybean by Dumas combustion method[J].China Oils and Fats,2019,44(1):.] |
| 杜马斯燃烧法测定大豆水溶性蛋白含量的方法研究 |
| Method for determination of water-soluble protein content in soybean by Dumas combustion method |
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| DOI： |
| 中文关键词: 大豆 水溶性蛋白 杜马斯燃烧法 凯氏定氮法 |
| 英文关键词:soybean water-soluble protein Dumas combustion method Kjeldahl method |
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| 中文摘要: |
| 通过优化大豆水溶性蛋白的提取条件，采用杜马斯燃烧定氮法，建立了一种大豆水溶性蛋白的测定方法，并探讨了杜马斯燃烧定氮仪的试验条件。结果表明，最佳试验条件为：20?℃恒温振荡提取，提取时固液比1∶?30，提取次数2次，提取时间40 min；提取液蒸发、干燥时吸取量3~4 mL，电热板温度150?℃；杜马斯燃烧定氮仪氧气流速300 mL/min，氧气因子1.4 mL/mg。在最佳试验条件下，本方法的试验精密度符合GB/T 27404—2008“检测方法确认过程中实验室变异系数（CV）”的技术要求，与现行凯氏定氮法的测定结果无显著性差异（P=0.89）。本方法属于环境友好型化学分析方法，可以满足大豆水溶性蛋白的无污染、快速、准确检测工作。 |
| 英文摘要: |
| By optimizing the extraction conditions of soybean water-soluble protein and using Dumas combustion method, an determination method of soybean water-soluble protein content was established, and the optimal experimental conditions of the Dumas combustion instrument were discussed. The results showed that the optimal extraction and drying conditions were obtained as follows: solid-liquid ratio 1∶?30 at constant temperature 20?℃, extraction times twice, extraction time 40 min, extract volume 3-4 mL when the extract evaporated and dried, and electric heating plate temperature 150 ?℃. The optimal experimental conditions of the Dumas combustion instrument were obtained as follows: oxygen flow rate 300 mL/min, and oxygen factor 1.4 mL/mg. Under the optimal conditions, the test precision of this method met the technical requirements of laboratory variation coefficient (CV) during the validation of the test method in GB/T 27404—2008, and there was no significant difference from the current Kjeldahl test method (P=0.89). The method belonged to the environment-friendly chemical analysis method and could meet the pollution-free, rapid and accurate detection of water-soluble protein in soybean. |
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