| 李云琴，陈中华，原晓龙，等.蒜头果中3-酮酯酰-CoA合酶基因克隆与表达分析[J].中国油脂,2019,44(3):.[LI Yunqin, CHEN Zhonghua, YUAN Xiaolong,etc.Cloning and expression analysis of 3-ketoacyl-CoA synthase gene from Malania oleifera[J].China Oils and Fats,2019,44(3):.] |
| 蒜头果中3-酮酯酰-CoA合酶基因克隆与表达分析 |
| Cloning and expression analysis of 3-ketoacyl-CoA synthase gene from Malania oleifera |
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| DOI： |
| 中文关键词: 蒜头果 3-酮酯酰-CoA合酶基因 荧光定量PCR 基因表达分析 |
| 英文关键词:Malania oleifera KCS gene RT-PCR gene expression analysis |
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| 中文摘要: |
| 以蒜头果(Malania oleifera)为实验材料，基于转录组数据分析，采用RT-PCR方法获得蒜头果3-酮酯酰-CoA合酶（KCS）基因的cDNA序列，命名为MoKCS1，GenBank登录号为MK210592。序列分析显示MoKCS1基因cDNA全长为1 539 bp,编码512个氨基酸，属于KCS家族。序列比对分析显示MoKCS1拥有KCS家族特有的3个功能保守结构域，与榴莲(Durio zibethinus)KCS的蛋白序列同源性为80.66%；与已知超长链KCS蛋白进行系统进化分析显示，MoKCS1独立形成一个分支。荧光定量PCR分析表明，MoKCS1在蒜头果果实膨大期的表达量最高，而在叶中几乎不表达。 |
| 英文摘要: |
| The Malania oleifera was used as material to clone the full length cDNA of the 3-ketoacyl-CoA synthase (KCS) gene based on the transcriptome of Malania oleifera with the method of RT-PCR technology, and named MoKCS1(GenBank ID: MK210592). The sequence analysis of MoKCS1 gene showed that the full-length cDNA of MoKCS1 gene was 1 539 bp, encoded 512 amino acids, and belonged to KCS family. The proteinic sequence alignment revealed that there was 80.66% of similarity between Malania oleifera and Durio zibethinus, and MoKCS1 had three functionally conserved domains that were unique in the KCS family. The phylogenetic analysis with known ultralong-chain KCS proteins revealed that no protein was found to be closely related to MoKCS1. The fluorescent quantitative RT-PCR certified that the expression of MoKCS1 gene in fruit of the flower within 3 months was the highest. |
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