| 董合磊1，吴颖峰1，刘 佳1，马小宏2，肖红梅1.响应面法优化酶法提取鳄鱼油工艺研究[J].中国油脂,2019,44(10):13~17.[DONG Helei1, WU Yingfeng1, LIU Jia1, MA Xiaohong2, XIAO Hongmei1.Optimization of enzymatic extraction of crocodile oil by response surface methodology[J].China Oils and Fats,2019,44(10):13~17.] |
| 响应面法优化酶法提取鳄鱼油工艺研究 |
| Optimization of enzymatic extraction of crocodile oil by response surface methodology |
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| DOI： |
| 中文关键词: 鳄鱼油 酶法提取 理化性质 脂肪酸组成 |
| 英文关键词:crocodile oil enzymatic extraction physicochemical property fatty acid composition |
| 基金项目:江苏省农业科技自主创新资金项目（CX173038） |
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| 中文摘要: |
| 以鳄鱼油提取率为评价指标，利用单因素实验和响应面法对酶法提取鳄鱼油工艺进行优化，并对鳄鱼油的理化性质和脂肪酸组成进行分析。结果表明：最佳酶法提取鳄鱼油工艺条件为中性蛋白酶添加量0.3%、提取时间3 h、料液比1∶ 0.45、提取温度54 ℃，在此条件下鳄鱼油提取率达到87.42%；鳄鱼油质量达到SC/T 3502—2016一级粗鱼油的标准；鳄鱼油中共检出32种脂肪酸，其中饱和脂肪酸含量为40.96%，不饱和脂肪酸含量为58.57%，EPA与DHA总量为2.42%。 |
| 英文摘要: |
| The extraction rate of crocodile oil was used as the evaluation index, and the single factor experiment and response surface methodology were used to optimize the process of enzymatic extraction of crocodile oil. And the physicochemical property and fatty acid composition of crocodile oil were analyzed. The results showed that the optimal enzymatic extraction conditions of crocodile oil were obtained as follows: neutral protease addition amount 0.3%, extraction time 3 h, ratio of material to liquid 1∶ 0.45, and extraction temperature 54 ℃. Under these conditions, the extraction rate of crocodile oil reached 8742%. The quality of crocodile oil reached the standard of SC/T 3502—2016 first grade crude fish oil. A total of 32 fatty acids were detected in crocodile oil, in which saturated fatty acid content, unsaturated fatty acid content and total content of EPA and DHA were 40.96%, 58.57% and 2.42%. |
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