| 邹燕娣，包李林，周青燕，熊巍林，张谦益，周双全.两种色谱柱分离37种脂肪酸组分研究[J].中国油脂,2019,44(10):156~160.[ZOU Yandi， BAO Lilin， ZHOU Qingyan， XIONG Weilin，
ZHANG Qianyi， ZHOU Shuangquan.Separation of 37 fatty acid components by two chromatographic columns[J].China Oils and Fats,2019,44(10):156~160.] |
| 两种色谱柱分离37种脂肪酸组分研究 |
| Separation of 37 fatty acid components by two chromatographic columns |
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| DOI： |
| 中文关键词: 气相色谱 色谱柱 脂肪酸组分 植物油 |
| 英文关键词:gas chromatography chromatographic column fatty acid component vegetable oil |
| 基金项目:湖南省重点研发计划（2017NK2212） |
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| 中文摘要: |
| 分别采用CD-2560色谱柱和DB-WAX色谱柱对37种脂肪酸标样和油样脂肪酸组分进行分离，探索了色谱柱流速、分流比和程序升温对分离效果的影响。利用已知CD-2560色谱柱对37种脂肪酸混标组分出峰顺序、标样质量浓度和油样比对，理顺了DB-WAX色谱柱测定37种脂肪酸混标中各组分出峰顺序和名称。结果表明，这两种色谱柱的最佳分离条件为：混标质量浓度1 mg/mL，柱流速0.55 mL/min，分流比70∶ 1，程序升温为100 ℃保持5 min，以3 ℃/min上升至230 ℃（CD-2560色谱柱保持30 min，DB-WAX色谱柱保持50 min）。采用这两种色谱柱用于5种油样中脂肪酸组分含量的测定，分离效果几乎一致，说明CD-2560色谱柱和DB-WAX色谱柱均能较好地应用于植物油中37种脂肪酸组分的测定。 |
| 英文摘要: |
| Thirty-seven fatty acid standard samples and oil samples were separated by CD-2560 chromatographic column and DB-WAX chromatographic column respectively. The effects of flow rate，split ratio and temperature programming on the separation effect of the chromatographic column were explored. In addition，the peak order and the name of each group in the 37 fatty acid mixtures separated by DB- WAX were determined by the known CD-2560 chromatographic column for the peak order，standard sample mass concentration and oil sample comparison. The results showed that the optimal separation conditions of these two kinds of chromatographic columns were obtained as follows: mass concentration of mixed standard 1 mg/mL, column flow rate 0.55 mL/min, split ratio 70∶ 1, temperature programmed from 100 ℃ (retaining for 5 min) to 230 ℃ at 3 ℃/min rate (CD-2560 chromatographic column retaining for 30 min, DB-WAX chromatographic column retaining for 50 min).Using these two chromatographic columns to determine the fatty acid content in five oil samples， the separation results were almost identical， which meant that the CD-2560 chromatographic column and the DB-WAX chromatographic column could be applied to determine 37 fatty acids in the vegetable oil. |
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