| 蒋忠荣，张涛，王涛，常明，刘睿杰，金青哲，王兴国.沙棘果油纳米乳液的性质与抗氧化活性研究[J].中国油脂,2019,44(12):59~64.[JIANG Zhongrong, ZHANG Tao, WANG Tao, CHANG Ming, LIU Ruijie,
JIN Qingzhe, WANG Xingguo.Properties and antioxidant activities of seabuckthorn fruit oil nanoemulsions JIANG Zhongrong, ZHANG Tao, WANG Tao, CHANG Ming, LIU Ruijie,[J].China Oils and Fats,2019,44(12):59~64.] |
| 沙棘果油纳米乳液的性质与抗氧化活性研究 |
| Properties and antioxidant activities of seabuckthorn fruit oil nanoemulsions JIANG Zhongrong, ZHANG Tao, WANG Tao, CHANG Ming, LIU Ruijie, |
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| DOI： |
| 中文关键词: 沙棘果油 纳米乳液 细胞毒性 抗氧化活性 |
| 英文关键词:seabuckthorn fruit oil nanoemulsion cytotoxicity antioxidant activity |
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| 中文摘要: |
| 以食品级的酪蛋白酸钠作为乳化剂，采用高压均质的方法制备沙棘果油纳米乳液。分析了5种沙棘果油样品的脂肪酸和主要脂质伴随物组成，并对其制得的沙棘果油纳米乳液特性及稳定性、细胞毒性、细胞抗氧化活性进行了研究。结果表明：沙棘果油主要脂肪酸包括棕榈油酸、棕榈酸、油酸和亚油酸，其中棕榈油酸含量高达32.23%~35.24%；总生育酚含量为188.28~244.18 mg/kg，植物甾醇含量为6 994.34~11 091.26 mg/kg，β-胡萝卜素含量为183.25~241.25 mg/kg；沙棘果油纳米乳液平均粒径范围在156.3~173.4 nm，PDI小于0.3，Zeta电位的范围在-50.51~-5795 mV，Ke小于15%，乳液体系均匀，稳定性良好；在高质量浓度情况下沙棘果油纳米乳液对HepG2细胞具有一定的抑制作用，半数抑制浓度（IC50）为1 443 μg/mL，并且100 μg/mL的5种沙棘果油纳米乳液的CAA值分别为171.88、307.71、23803、255.24、310.54 μmolQE/g，除1号样品外，均优于相同质量浓度的橄榄油纳米乳液（CAA值为222.89 μmolQE/g），表现出良好的细胞抗氧化活性。 |
| 英文摘要: |
| Food-grade sodium caseinate was used as emulsifier to prepare seabuckthorn fruit oil nanoemulsions using high-pressure homogenization method. The composition of fatty acid and lipid concomitant of five seabuckthorn fruit oil samples were analyzed, and the emulsion property, stability, cytotoxicity and cellular antioxidant activity of the seabuckthorn fruit oil nanoemulsions were studied. The results showed that the main fatty acids of the seabuckthorn fruit oils were palmitoleic acid, palmitic acid, oleic acid and linoleic acid, and the palmitoleic acid content was 32.23%-35.24%. The contents of total tocopherol, phytosterol and β-carotene were 188.28-244.18 mg/kg, 6 994.34-11 091.26 mg/kg and 183.25-241.25 mg/kg, respectively. The average particle size, PDI, Zeta potential and Ke were 156.3-173.4 nm,less than 0.3, -50.51--57.95 mV and less than 15%, respectively. The obtained emulsion system was uniform and stable, and the seabuckthorn fruit oil nanoemulsions had a certain inhibitory effect on HepG2 cells at high mass concentration, and the cell viability was 50% when the treatment mass concentration reached 1 443 μg/mL. The cellular antioxidant activities value (CAA) of 100 μg/mL five seabuckthorn fruit oil nanoemulsions samples were 171.88, 307.71, 238.03, 25524, 31054 μmolQE/g, respectively. Except for sample 1, the other four seabuckthorn fruit oil nanoemulsions exhibited better cellular antioxidant activity than olive oil nanoemulsions (CAA was 222.89 μmolQE/g) of the same mass concentration. |
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