| 陶林，邓泽元，范亚苇，李静.反棕榈油酸对人肝细胞脂质的影响[J].中国油脂,2019,44(12):97~103.[TAO Lin，DENG Zeyuan，FAN Yawei，LI Jing.Effect of trans palmitoleic acid on lipid in human hepatocytes[J].China Oils and Fats,2019,44(12):97~103.] |
| 反棕榈油酸对人肝细胞脂质的影响 |
| Effect of trans palmitoleic acid on lipid in human hepatocytes |
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| DOI： |
| 中文关键词: 反棕榈油酸 脂肪变性 人肝细胞 细胞脂质 氧化 |
| 英文关键词:trans palmitoleic acid steatosis human hepatocytes cell lipid oxidation |
| 基金项目:江西省杰出青年人才资助计划（20171BCB23024） |
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| 中文摘要: |
| 流行病学研究报道反刍动物代表性反式脂肪酸反棕榈油酸（9t16∶ 1）具有降低血脂的作用，为了进一步研究9t16∶ 1对细胞脂质代谢的影响，采用不同浓度反棕榈油酸作用于脂肪变性人肝细胞LO2，通过测定细胞存活率、细胞脂肪酸组成、脂质含量变化以及细胞氧化情况反映反棕榈油酸对脂质代谢的作用。结果表明：100 μmol/L的9t16∶ 1作用于人肝细胞后饱和脂肪酸含量降低了3489%，单不饱和脂肪酸含量升高了16.44%，多不饱和脂肪酸含量升高了30.50%；9t16∶ 1在人肝细胞内部分生物转化为11t18∶ 1和CLA，其转化率分别为17.50%和16.42%；9t16∶ 1显著降低脂肪变性人肝细胞甘油三酯和胆固醇含量，降低率分别为61.76%和37.80%；9t16∶ 1显著升高人肝细胞MDA水平（升高率为45.36%）和降低SOD活力（降低74.42%），促进细胞内脂质氧化。因此，反棕榈油酸可通过促进人肝细胞脂质氧化达到降低脂质堆积的作用。 |
| 英文摘要: |
| Epidemiological study has reported that trans palmitoleic acid (9t16∶ 1),the representative trans fatty acid in ruminants,has the effect of reducing blood lipid. To further investigate the effect of 9t16∶ 1 on lipid metabolism in cells,different concentrations of 9t16∶ 1 were adopted to treat steatotic human hepatocytes LO2 in the experiment, and the indexes of the cell viability, intracellular fatty acid composition, lipid content changes and cell lipid oxidation were determined. The results showed that the content of saturated fatty acids decreased by 34.89%, the content of monounsaturated fatty acids increased by 16.44% and the content of polyunsaturated fatty acids increased by 30.50% after human hepatocytes being treated with 9t16∶ 1 of 100 μmol/L.9t16∶ 1 Partly transformed into 11t18∶ 1 and CLA in human hepatocytes, and the conversion rates were 17.50% and 16.42% respectively.9t16∶ 1 Significantly reduced the contents of triglyceride and cholesterol in steatotic human hepatocytes with the reduction rates of 61.76% and 37.80%, respectively.In addition, 9t16∶ 1 promoted intracellular lipid oxidation by significantly increasing the MDA level(increased by 45.36%) and decreasing SOD activity (decreased by 74.42%)in human hepatocytes.Therefore, 9t16∶ 1 could reduce lipid accumulation by promoting lipid oxidation in human hepatocytes. |
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