| 郝广萍.基于斑马鱼帕金森模型研究牡丹籽油微囊粉的抗帕金森病活性[J].中国油脂,2020,45(8):45~50.[HAO Guangping.Anti-Parkinsons activity of peony seed oil microcapsule powder based on zebrafish Parkinsons desease model[J].China Oils and Fats,2020,45(8):45~50.] |
| 基于斑马鱼帕金森模型研究牡丹籽油微囊粉的抗帕金森病活性 |
| Anti-Parkinsons activity of peony seed oil microcapsule powder based on zebrafish Parkinsons desease model |
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| DOI：10.12166/j.zgyz.1003-7969/2020.08.010 |
| 中文关键词: 牡丹籽油 牡丹籽油微囊粉 斑马鱼 帕金森病 |
| 英文关键词:：peony seed oil peony seed oil microcapsule powder zebrafish Parkinsons desease |
| 基金项目:山东省重点研发计划（2017YYSP032）；山西医科大学汾阳学院人才引进启动金基金项目（2016D03） |
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| 中文摘要: |
| 为了探究牡丹籽油微囊粉是否具有抗帕金森病活性，实验分正常对照组、50 μmol/L MPTP处理组、50 μmol/L MPTP与不同质量浓度（25、50、100 μg/mL）牡丹籽油微囊粉共处理组，将受精后1 d的转基因斑马鱼Vmat:GFP、Fli1:GFP胚胎和野生型斑马鱼AB胚胎做对应处理。受精后3 d时荧光显微镜观察并记录转基因斑马鱼Vmat:GFP （n=8）多巴胺神经元的发育情况和转基因斑马鱼Fli1:GFP（n=8）脑部血管的发育情况；受精后5 d时用Zebrabox斑马鱼行为分析仪分析野生型斑马鱼AB（n=8）行为，通过qRT-PCR检测野生型斑马鱼AB（n=40）帕金森病相关基因(α-syn、parkin、pink1)的表达变化。结果显示：与正常对照组比较，MPTP处理组的斑马鱼多巴胺神经元缺失，脑部血管损伤，出现帕金森病行为，帕金森病相关基因表达异常；与MPTP处理组比较，不同质量浓度的牡丹籽油微囊粉与MPTP共处理组的斑马鱼表现出多巴胺神经元缺失减少，脑部血管损伤降低，帕金森病行为缓解，相关基因异常表达趋于正常，且随着牡丹籽油微囊粉质量浓度的增加，其效果愈加显著。结果表明，牡丹籽油微囊粉具有抗帕金森病活性，并具有剂量依赖性。 |
| 英文摘要: |
| In order to investigate whether peony seed oil microcapsule powder had anti-Parkinsons activity, different experiment groups were set including the control group, 50 μmol/L MPTP treatment group, and co-treatment groups (25, 50,100 μg/mL) of peony seed oil microcapsule powder with 50 μmol/L MPTP. Transgenic zebrafish Vmat:GFP, Fli1:GFP embryos, and wild-type zebrafish AB embryos were treated at 1 day after fertilization(1 dpf). At 3 dpf, the development of dopaminergic neurons of transgenic zebrafish Vmat:GFP (n=8) and the brain blood vessels development of transgenic zebrafish Fli1:GFP (n=8) were observed and recorded by fluorescence. At 5 dpf, the behavior of wild-type zebrafish AB (n=8) was analyzed by Zebrabox zebrafish behavior analyzer, and the expression of genes (α-syn、parkin、pink1)involved in Parkinsons desease(PD)in wild-type zebrafish AB (n=40) was detected by qRT-PCR. Compared with the control group, MPTP treatment group exhibited loss of dopaminergic neurons, damaged blood vessels in the brain, existence of PD-like behavior, and abnormal expression of PD-related genes. Compared with the MPTP treatment group, co-treatment groups reduced dopaminergic neurons loss and the damage of blood vessels in the brain, as well as relieved PD-like behavior and abnormal expression of PD-related genes, and these effects were strengthened with the increase of peony seed oil concentration. The results showed that peony seed oil microcapsule powder had anti-Parkinsons disease activity and was dose-dependent. |
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