| 王肖行.美藤果油体外抗氧化性能研究[J].中国油脂,2021,46(4):63~67.[WANG Xiaohang.In vitro antioxidant activity of Sacha Inchi oil[J].China Oils and Fats,2021,46(4):63~67.] |
| 美藤果油体外抗氧化性能研究 |
| In vitro antioxidant activity of Sacha Inchi oil |
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| DOI： |
| 中文关键词: 美藤果油 自由基清除率 还原能力 抗氧化活性 |
| 英文关键词:Sacha Inchi oil free radical scavenging rate reducing capacity antioxidant activity |
| 基金项目:云南省重大科技专项计划（2018ZG003） |
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| 中文摘要: |
| 以美藤果油为研究对象，TBHQ为阳性对照，采用典型的体外抗氧化试验体系测定了美藤果油对DPPH·、·OH、O-2·的清除能力，Fe2+的螯合能力以及还原能力，用于评价美藤果油的体外抗氧化性能。结果表明：美藤果油的体外抗氧化性能与其质量浓度之间存在良好的剂量效应关系；在试验质量浓度范围内，美藤果油5种体外抗氧化指标的最大值分别为DPPH·清除率75.24%、·OH清除率95.26%、O-2·清除率92.57%、Fe2+螯合率92.17%和还原能力1.22；在质量浓度相同的情况下，美藤果油对O-2·的清除能力和Fe2+的螯合能力优于TBHQ；美藤果油对DPPH·、·OH 和O-2·的清除能力以及Fe2+螯合能力的IC50分别为2.96、<0.01、0.20、2.77 mg/mL。研究结果表明美藤果油具有较强的体外抗氧化性能。 |
| 英文摘要: |
| In order to investigate in vitro antioxidant activity of Sacha Inchi oil, the typical in vitro antioxidant test system was used to determine the scavenging ability of Sacha Inchi oil on DPPH·,·OH, O-2·, capacity of chelating Fe2+ and reducing capacity, with TBHQ as positive control. The results indicated that there was a good dose-response relationship between the in vitro antioxidant properties and the mass concentration of Sacha Inchi oil. Within the range of the test mass concentration, the maximum values of the five in vitro antioxidant indexes of Sacha Inchi oil were DPPH· scavenging rate 75.24%, ·OH scavenging rate 95.26%, O-2·scavenging rate 92.57%, capacity of chelating Fe2+92.17% and reducing capacity 1.22, respectively. In the case of the same mass concentration, the O-2· scavenging ability and capacity of chelating Fe2+ of Sacha Inchi oil were better than those of TBHQ. The IC50 of DPPH· scavenging ability, ·OH scavenging ability, O-2· scavenging ability and capacity of chelating Fe2+ of Sacha Inchi oil were 2.96, below 0.01, 0.20, 2.77 mg/mL，respectively. The above results showed that Sacha Inchi oil had stronger in vitro antioxidant activity. |
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