| 甘欢华1，张斌2，刘钟栋1，李华1，魏国华2.气相色谱法同时快速测定大豆油脱臭馏出物中的生育酚、植物甾醇和角鲨烯含量[J].中国油脂,2021,46(5):.[GAN Huanhua1，ZHANG Bin2，LIU Zhongdong1，LI Hua1，WEI Guohua2.Simultaneous and rapid determination of tocopherol, phytosterol and squalene in soybean oil deodorized distillate by gas chromatography[J].China Oils and Fats,2021,46(5):.] |
| 气相色谱法同时快速测定大豆油脱臭馏出物中的生育酚、植物甾醇和角鲨烯含量 |
| Simultaneous and rapid determination of tocopherol, phytosterol and squalene in soybean oil deodorized distillate by gas chromatography |
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| DOI： |
| 中文关键词: 关键词：大豆油脱臭馏出物 气相色谱法 同时测定 生育酚 植物甾醇 角鲨烯 |
| 英文关键词:Key words：soybean oil deodorized distillate gas chromatography simultaneous determination tocopherol phytosterol squalene |
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| 中文摘要: |
| 摘要：建立了可以同时定量分析大豆油脱臭馏出物中生育酚（α-生育酚、(β+γ)-生育酚、δ-生育酚）、植物甾醇（菜籽甾醇、菜油甾醇、豆甾醇、β-谷甾醇）和角鲨烯含量的气相色谱检测方法。以十六酸十六醇酯为内标物质，样品直接溶解在内标溶液中，采用HP-1色谱柱分离，以内标法定量。结果表明：各组分之间分离度良好，生育酚、植物甾醇和角鲨烯分别在0.4~6.4 mg/mL、0.5~8.0 mg/mL、0.4~4.0 mg/mL范围内呈现良好的线性关系，R2均大于等于0.999，平均加标回收率分别为95.38%~102.78%、96.40%~101.24%、96.14%~104.43%，相对标准偏差均小于10%，检出限分别为0.08、0.15、0.05 mg/L。该方法前处理简单、重复性好、灵敏度高、结果准确可靠，可应用于大豆油脱臭馏出物中生育酚、植物甾醇、角鲨烯含量的同时测定。 |
| 英文摘要: |
| Abstract：A gas chromatography detection method was established, which could simultaneously quantitatively analyze the tocopherols (α-tocopherol, (β+γ)-tocopherol, δ-tocopherol),phytosterols (rapeseed sterol, campesterol, stigmasterol, β-sitosterol) and squalene in soybean oil deodorized distillate. With cetyl palmitate as the internal standard substance, the sample was directly dissolved in the internal standard solution, and the HP-1 column was used to separate various components. The results showed that the separation of various components was good. The tocopherol, phytosterol and squalene showed good linear relationships in the ranges of 0.4-6.4 mg/mL, 0.5-8.0 mg/mL and 0.4-4.0 mg/mL respectively, the relative coefficients were all greater than or equal to 0.999, and their average recovery rates were 95.38%-102.78%,96.40%- 101.24%,96.14%-104.43% respectively. The relative standard deviations were all lower than 10%, and their detection limits were 0.08, 0.15 mg/L and 0.05 mg/L, respectively. The method had the items of simple pretreatment, good reproducibility, high sensitivity, and accurate and reliable results, which could be applied in the simultaneous determination of tocopherol, phytosterol and squalene in soybean oil deodorized distillate. |
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