| 王凌翌.联合酶法提取豆渣蛋白肽和可溶性膳食纤维[J].中国油脂,2021,46(6):114~118.[WANG Lingyi.Extraction of protein peptides and soluble dietary fiber from soybean dregs by combined enzymatic method[J].China Oils and Fats,2021,46(6):114~118.] |
| 联合酶法提取豆渣蛋白肽和可溶性膳食纤维 |
| Extraction of protein peptides and soluble dietary fiber from soybean dregs by combined enzymatic method |
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| DOI： |
| 中文关键词: 豆渣 膳食纤维 蛋白肽 酶解 |
| 英文关键词:soybean dregs dietary fiber protein peptide enzymolysis |
| 基金项目:贵港市科学研究与技术开发计划项目(贵科攻1829011) |
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| 中文摘要: |
| 豆渣是大豆加工的主要副产物之一，含有丰富的蛋白质和膳食纤维。为促进豆渣高值化利用，采用联合酶法从豆渣中提取蛋白肽和可溶性膳食纤维（SDF）。首先用碱性蛋白酶酶解豆渣蛋白，以蛋白肽得率为指标，通过单因素试验优化了提取豆渣蛋白肽的工艺条件，再将脱蛋白豆渣用纤维素酶酶解制备SDF，以SDF提取率为指标，通过单因素试验优化提取SDF的工艺条件。结果表明：碱性蛋白酶酶解提取蛋白肽最佳工艺条件为料液比1∶ 35、酶与底物比2%、酶解时间5 h、酶解温度50 ℃、pH 95，在此条件下豆渣蛋白肽得率为66.81%；纤维素酶酶解提取SDF最佳工艺条件为料液比1∶ 30、酶与底物比3%、酶解温度50 ℃、酶解时间2 h、pH 4.0，在此条件下SDF提取率为1554%。利用碱性蛋白酶和纤维素酶依次酶解后，豆渣总利用率达到了89.81%，这为豆渣综合开发利用提供了一种新途径。 |
| 英文摘要: |
| Soybean dregs are one of the main by-products of soybean processing, and are rich in protein and dietary fiber. The protein peptides and soluble dietary fiber (SDF) were separately extracted from soybean dregs by combined enzymatic method. First, the protein in the soybean dregs was hydrolyzed with alcalase, and the yield of protein peptides was used as an indicator,the conditions for extracting protein peptides from soybean dregs were optimized through single factor experiment, and then the deproteinized soybean dregs were hydrolyzed with cellulase to prepare SDF, and the yield of SDF was used as an indicator, the process conditions for extracting SDF were optimized through single factor experiment. The results showed that the optimal conditions for extracting protein peptides from soybean dregs were obtained as follows: material-liquid ratio 1∶ 35, enzyme-substrate ratio (E/S)2%, enzymolysis temperature 50 ℃, pH 9.5, and enzymolysis time 5 h. Under the optimal conditions,the yield of protein peptides was 66.81%.The optimal conditions for extracting SDF from deproteinized soybean dregs were obtained as follows: material-liquid ratio 1∶ 30, E/S 3%, enzymolysis temperature 50 ℃, enzymolysis time 2 h, and pH 40. Under the optimal conditions,the yield of SDF was 1554%. After enzymolysis with alcalase and cellulase sequentially, the total utilization rate of soybean dregs reached 89.81%, which provided a new way for the comprehensive development and utilization of soybean dregs. |
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