| 周昊.响应面法优化漆籽油提取工艺 及其抗氧化活性研究[J].中国油脂,2021,46(9):5~10.[ZHOU Hao.Optimization of extraction technology of lacquer seed oil by response surface methodology and its antioxidant activity[J].China Oils and Fats,2021,46(9):5~10.] |
| 响应面法优化漆籽油提取工艺 及其抗氧化活性研究 |
| Optimization of extraction technology of lacquer seed oil by response surface methodology and its antioxidant activity |
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| DOI： |
| 中文关键词: 漆籽油 溶剂提取 响应面优化 理化性质 脂肪酸组成 抗氧化活性 |
| 英文关键词:lacquer seed oil solvent extraction response surface methodology optimization physico chemical index fatty acid composition antioxidant activity |
| 基金项目:中国林科院基本科研业务费专项资金(CAFYBB2018ZB009) |
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| 摘要点击次数: 818 |
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| 中文摘要: |
| 以秦巴山区漆籽为原料，采用溶剂（石油醚）浸提法从其种仁中提取漆籽油，在单因素试验的基础上，采用响应面法优化漆籽油提取工艺，对漆籽油的理化性质进行分析，采用气质联用分析漆籽油的脂肪酸组成，并评价其抗氧化活性。结果表明：漆籽油的最优提取工艺条件为提取温度70 ℃、提取时间60 min、液料比10∶ 1、提取次数2次，在此条件下漆籽油提取率为9614%；漆籽油相对密度为0.925 8，折光指数(20 ℃)为1.467 2，碘值(I)为146.83 g/100 g，酸值( KOH)为2.58 mg/g，过氧化值为3.62 mmol/kg，皂化值(KOH)为185.36 mg/g；漆籽油主要含有油酸、亚油酸、棕榈酸、硬脂酸、亚麻酸和棕榈油酸6种脂肪酸，不饱和脂肪酸含量达86.10%，以油酸和亚油酸为主，二者含量达到82.17%；漆籽油对DPPH、ABTS及羟自由基的半数清除浓度(IC50)分别为35.38、41.29、38.52 mg/L，具有与维生素C相当的抗氧化活性。 |
| 英文摘要: |
| Lacquer seed oil was extracted from kernel of lacquer seeds from Qinba Mountain by solvent(petroleum ether) extraction.On the basis of single factor experiment, response surface methodology was used to optimize the extraction process of lacquer seed oil. The physicochemical indexes of lacquer seed oil were analyzed,and the fatty acid composition of lacquer seed oil was analyzed by GC-MS, its antioxidant activity was also evaluated. The results showed that the optimal extraction process of lacquer seed oil were obtained as follows: extraction temperature 70 ℃, extraction time 60 min, liquid-material ratio 10∶ 1, extraction times twice.Under the optimal conditions,the extraction rate of lacquer seed oil was 96.14%.The relative density, refractive index (20 ℃), iodine value,acid value, peroxide value and saponification value of lacquer seed oil were 0.925 8, 1.467 2, 146.83 gI/100 g, 2.58 mgKOH/g, 3.62 mmol /kg and 185.36 mgKOH/g, respectively. Lacquer seed oil mainly contained six kinds of fatty acids including oleic acid, linoleic acid, palmitic acid, stearic acid, linolenic acid and palmitoleic acid, and the content of unsaturated fatty acids reached up to 86.10%, in which the content of oleic acid and linoleic acid reached 82.17%. The IC50 of lacquer seed oil for DPPH, ABTS and hydroxyl radicals were 35.38, 41.29 mg/L and 38.52 mg/L, showing equivalent antioxidant activity with vitamin C. |
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