| 龙芳.汉麻肽钙螯合物的制备及其结构表征和稳定性[J].中国油脂,2021,46(9):33~39.[LONG Fang.Preparation of hemp peptide-calcium chelate and its structural characterization and stability properties[J].China Oils and Fats,2021,46(9):33~39.] |
| 汉麻肽钙螯合物的制备及其结构表征和稳定性 |
| Preparation of hemp peptide-calcium chelate and its structural characterization and stability properties |
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| DOI： |
| 中文关键词: 汉麻肽 肽钙螯合物 结构表征 稳定性 |
| 英文关键词:hemp peptide peptide calcium chelate structure characterization stability |
| 基金项目:湖南省教育厅科学研究项目（18C1827） |
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| 中文摘要: |
| 汉麻籽仁经脱脂、提取汉麻蛋白、酶法水解制备汉麻肽（HPIH），再与钙螯合制备汉麻肽钙螯合物（HPIH-Ca）。以钙螯合率为指标，采用单因素实验和正交实验优化HPIH-Ca制备工艺条件；运用红外光谱和圆二色谱分析HPIH-Ca的结构，利用扫描电镜表征HPIH-Ca微观结构，通过热处理、pH和体外模拟消化评价HPIH-Ca的稳定性。结果表明：胰酶是最佳水解酶；HPIH-Ca的最佳制备工艺条件为肽钙比2∶ 1（10 mg/mL HPIH溶液与0.05 mol/L无水氯化钙溶液体积比）、pH 8.0、反应温度60 ℃、反应时间45 min，在最佳条件下钙螯合率为47.61 mg/g；Ca2+与汉麻肽主要通过羧基氧原子和氨基氮原子相互作用； Ca2+与HPIH结合后形成了更为有序和紧凑的二级结构；HPIH与Ca2+交联形成结构有序、紧密的呈片状或大颗粒的肽钙螯合物；HPIH-Ca具有一定的耐热性，但在酸性条件下不稳定；体外模拟消化实验结果显示，HPIH-Ca能保持70%以上的持钙率，具有较高的生物利用度。 |
| 英文摘要: |
| Hemp peptide(HPIH) was prepared by defatting, protein extraction and enzymatic hydrolysis of hemp seed kernel, then chelated with calcium to prepare hemp peptide-calcium chelate(HPIH-Ca), and the calcium chelation rate was used as the index to optimize preparation parameters by single factor experiment and orthogonal experiment. The structure of the chelate was analyzed by infrared spectroscopy and circular dichroism, and the microstructure of the chelate was characterized by scanning electron microscopy. The stability of the chelate was evaluated by heat treatment, pH and simulated digestion in vitro. The results showed that trypsin was the best hydrolase, and the optimal process parameters were obtained as follows: ratio of peptide to calcium 2∶ 1 (volume ratio of 10 mg/mL HPIH solution to 0.05 mol/L anhydrous calcium chloride), pH 8.0, reaction temperature 60 ℃ and reaction time 45 min. Under these conditions, calcium chelation rate was 47.61 mg/g. The interaction between Ca2+ and hemp peptide was mainly through carboxylic oxygen and amino nitrogen atoms. The Ca2+ combined with HPIH to form a more ordered and compact secondary structure. HPIH was cross-linked with Ca2+ to form a large granular and lamellar complex with orderly and tight structure. HPIH-Ca had certain heat resistance but was unstable under acidic conditions. Simulated digestion in vitro results showed that HPIH-Ca chelate could maintain calcium retention rate of more than 70% and had high bioavailability. |
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