| 崔茂凯1,2，黄海涛3，李庆杨1，沈丹玉1，肖鹏飞3，刘毅华1.核桃仁内种皮中缩合单宁多聚体的分离
及其抗氧化活性分析[J].中国油脂,2025,50(4):.[CUI Maokai1,2, HUANG Haitao3, LI Qingyang1, SHEN Danyu1,
XIAO Pengfei3, LIU Yihua1.Isolation and antioxidant activity analysis of condensed tannin polymers in walnut pellicle[J].China Oils and Fats,2025,50(4):.] |
| 核桃仁内种皮中缩合单宁多聚体的分离
及其抗氧化活性分析 |
| Isolation and antioxidant activity analysis of condensed tannin polymers in walnut pellicle |
| |
| DOI：10.19902/j.cnki.zgyz.1003-7969.230612 |
| 中文关键词: 核桃 内种皮 缩合单宁 多聚体 抗氧化活性 |
| 英文关键词:walnut pellicle condensed tannins polymer antioxidant activity |
| 基金项目:中央级公益性科研院所基本科研业务费专项资金（CAFYBB2019QD002） |
|
| Author Name | Affiliation | | CUI Maokai1,2, HUANG Haitao3, LI Qingyang1, SHEN Danyu1,
XIAO Pengfei3, LIU Yihua1 | 1.Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China
2.School of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China
3.School of
Biology and Environment, Jiyang College of Zhejiang Agriculture and Forestry University, Shaoxing
312000, Zhejiang, China |
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| 中文摘要: |
| 为给核桃资源的高值化利用提供参考，以核桃仁内种皮为原料，通过70%甲醇溶液（含0.5%冰乙酸）萃取，Sephadex LH-20 葡聚糖凝胶层析柱分离，经不同体积分数的甲醇溶液梯度洗脱得到13个核桃仁内种皮缩合单宁多聚体分离物（F1~F13），以VC为标样，对这13个分离物的体外抗氧化活性进行分析。结果表明：采用甲醇溶液（体积分数40%~75%）梯度洗脱方法可从核桃仁内种皮中分离出缩合单宁二聚体（DP2）至七聚体（DP7）；DP2和缩合单宁三聚体（DP3）的洗脱甲醇溶液体积分数在40%~60%，缩合单宁四聚体（DP4）和其他3种高聚体的洗脱甲醇溶液体积分数在60%~75%；优化洗脱操作后 DP2 和 DP3可被高效分离，DP2洗脱率超过80%，其中F2、F3和F4中得到DP2，总含量超过1.40 mg/g，F5 和 F6中得到 DP3，含量分别为 0.66 mg/g和 0.59 mg/g；F1~F13都具有良好的抗氧化活性，其中以DP2为主的F4抗氧化活性最高，铁离子还原能力（FRAP） 和 DPPH自由基清除能力分别为（17.22±0.68） mg/g和（24.36±0.73） mg/g。综上，采用梯度洗脱方法可实现缩合单宁多聚体的洗脱分离，可从核桃仁内种皮中分离出 DP2和DP3。 |
| 英文摘要: |
| To provide a reference for the high-value utilization of walnut resources, walnut pellicle was used as raw material and extracted with a 70% methanol solution (containing 0.5% acetic acid). The extract was separated with a Sephadex LH-20 dextran gel column and subjected to gradient elution with different volume fractions of methanol solution to obtain 13 fractions of condensed tannin polymers (F1-F13). The in vitro antioxidant activity of these 13 fractions were analyzed using vitamin C as the standard sample. The results indicated that the gradient elution method with methanol solution (volume fraction 40%-75%) could successfully separate condensed tannin dimers (DP2) to heptamers (DP7) from walnut pellicle. The elution volume fractions for condensed DP2 and trimers (DP3) ranged from 40% to 60%, while the elution volume fractions for tetramers (DP4) and three other higher polymers were between 60% and 75%. After optimizing the elution process, efficient separation of DP2 and DP3 was achieved, with the elution rate of DP2 exceeding 80%. DP2 was isolated from elution fractions F2, F3, and F4, with a tatal content exceeding 1.40 mg/g, while DP3 was obtained from fractions F5 and F6, with contents of 0.66 mg/g and 0.59 mg/g, respectively. All fractions exhibited good antioxidant activity, with F4, primarily containing DP2, showing the highest antioxidant activity, measured by ferric ion reducing ant ioxidant power (FRAP) and DPPH methods as (17.22±0.68) mg/g and (24.36±0.73) mg/g, respectively. In conclusion, the gradient elution method enables the effective separation of condensed tannin polymers, allowing for the isolation of DP2 and DP3 from walnut pellicle. |
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