| 张鑫，凤元邦，冯雪，张春枝，陈明.紫外-LiCl联合ARTP诱变选育高产花生四烯酸的
高山被孢霉突变株[J].中国油脂,2025,50(4):.[ZHANG Xin, FENG Yuanbang, FENG Xue, ZHANG Chunzhi, CHEN Ming.Breeding of Mortierella alpina mutants with high yield of arachidonic acid by UV-LiCl combined with ARTP mutagenesis[J].China Oils and Fats,2025,50(4):.] |
| 紫外-LiCl联合ARTP诱变选育高产花生四烯酸的
高山被孢霉突变株 |
| Breeding of Mortierella alpina mutants with high yield of arachidonic acid by UV-LiCl combined with ARTP mutagenesis |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240040 |
| 中文关键词: 花生四烯酸；高山被孢霉；诱变育种；紫外-LiCl；ARTP
中图分类号：TQ92 TS201.3 文献标识码：A |
| 英文关键词:arachidonic acid Mortierella alpina mutagenesis breeding UV-LiCl ARTP |
| 基金项目:辽宁省自然科学基金（2024-MS-171） |
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| 摘要点击次数: 36 |
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| 中文摘要: |
| 为得到遗传性状稳定、高产花生四烯酸（ARA）的高山被孢霉菌株，以实验室保藏的高山被孢霉FD-1为出发菌株，采用紫外-LiCl联合常压室温等离子体（ARTP）技术进行诱变处理，并进行TTC平板初筛和摇瓶发酵复筛。结果表明，经紫外-LiCl联合ARTP诱变处理，获得一株高产ARA的高山被孢霉突变株Z6-A23。菌株Z6-A23所产油脂中ARA含量高达44.57%，ARA产量达3.62 g/L，分别较出发菌株FD-1提高了75.40%和223.21%。经10次传代培养，突变株Z6-A23代谢合成ARA性能稳定，说明其具有良好的遗传稳定性。综上，突变株Z6-A23有望成为高产ARA的潜力菌株。 |
| 英文摘要: |
| In order to obtain a genetically stable and high yield arachidonic acid (ARA)-producing Mortierella alpina strain, mutagenesis of the strain of M. alpina FD-1 preserved in laboratory was performed by UV-LiCl combined with atmospheric and room temperature plasma (ARTP) method, and the strain was screened by TTC plate and the fermentation in shaking flasks. The results showed that a mutant strain Z6-A23 with high yield of ARA was obtained by UV-LiCl combined with ARTP mutagenesis. The ARA content in the oil produced by the mutant strain Z6-A23 reached as high as 44.57%, and the ARA yield was 3.62 g/L, which were 75.40% and 223.21% higher than those of the original strain FD-1, respectively. After 10 subcultures, the mutant Z6-A23 had stable ability to synthesize ARA, indicating that it had good genetic stability. In conculsion, the mutant strain Z6-A23 obtained is expected to be a promising strain for efficient ARA production. |
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